东北大学学报（自然科学版）

东北大学学报(自然科学版) ›› 2012, Vol. 33 ›› Issue (7): 1060-1064.DOI: -

• 论著 • 上一篇    

转基因大麦茎秆木质素含量及相关特性

孙彩霞;吴晓菲;王杰;陈振华;   

  1. 东北大学理学院;中国科学院沈阳应用生态研究所;
  • 收稿日期:2013-06-19 修回日期:2013-06-19 发布日期:2013-04-04
  • 通讯作者: -
  • 作者简介:-
  • 基金资助:
    中央高校基本科研业务费专项资金资助项目(N090405011);;

Lignin content and related characteristics of transgenic barley stems

Sun, Cai-Xia (1); Wu, Xiao-Fei (1); Wang, Jie (1); Chen, Zhen-Hua (2)   

  1. (1) School of Sciences, Northeastern University, Shenyang 110819, China; (2) Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
  • Received:2013-06-19 Revised:2013-06-19 Published:2013-04-04
  • Contact: Sun, C.-X.
  • About author:-
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摘要： 以不同转基因大麦(T1,T2)及非转基因对照(WT,NT1)为供试对象,采用比色法及组织化学定位的显微分析并结合傅里叶红外光谱分析(FT-IR),研究了其茎秆木质素含量、代谢关键酶活性、茎秆解剖结构及FT-IR特征.结果表明:不同基因型大麦茎秆木质素含量、薄壁组织及维管束的数目、细胞壁中愈创木基木质素(G)、紫丁香基木质素(S)及A(S)/A(G)(吸收峰面积比)均未见显著改变.与野生型大麦比较,转基因大麦T1的木质素代谢关键酶苯丙氨酸解氨酶(PAL)活性的显著下调(降幅达66%)、过氧化物酶(POD)活性的显著上调(增幅达76%)、茎秆厚壁组织明显变薄及对-羟基苯基木质素(H)比例的显著减小...

关键词: 转基因大麦, 木质素, 酶活性, 解剖结构, FT-IR

Abstract: Taking different transgenic barley as test materials for pot experiment, the lignin content, key enzyme activity, stem structure and Fourier transform infrared spectroscopy of different barley stems were studied to reveal the unintended changes related to lignin metabolism in transgenic barley. The results showed that the lignin content, parenchymal tissue, numbers of vascular bundles, guaiacyl lignin (G), syringyl lignin (S) and A(S)/A(G) of transgenic barley (T1, T2) stems had no significant changes in comparison with that of the wild type (WT) and non-transgenic barley (NT1). The unintended changes of the transgenic barley T1, such as the key enzyme activities of lignin metabolism (for PAL decreased by 66% and for POD increased significantly by 76%), the sclerenchymal tissue and the ρ-hydroxyphenyl lignin (H) ratio (decreased by 59%), may be mainly caused by somaclonal variation generated from tissue culture during genetic transformation.

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