面向血管芯片微通道的红细胞形变学研究

doi:10.12068/j.issn.1005-3026.2023.06.019

东北大学学报（自然科学版） ›› 2023, Vol. 44 ›› Issue (6): 906-912.DOI: 10.12068/j.issn.1005-3026.2023.06.019

• 生物工程 • 上一篇

面向血管芯片微通道的红细胞形变学研究

胡晟^1,2，叶俊彦¹，赵勇^1,2

(1. 东北大学秦皇岛分校控制工程学院，河北秦皇岛066004；2. 东北大学秦皇岛分校河北省微纳精密光学传感与检测技术重点实验室，河北秦皇岛066004)

发布日期:2023-06-20
通讯作者: 胡晟
作者简介:胡晟(1984-)，男，云南景洪人，东北大学秦皇岛分校副教授.
基金资助:
国家自然科学基金资助项目(61903069)；河北省自然科学基金资助项目(F2020501040)；中央高校基本科研业务费专项资金资助项目(N2223034).

Study on Morphology of Red Blood Cell in Micro-channel Towards Vessel-on-Chip

HU Sheng^1,2， YE Jun-yan¹， ZHAO Yong^1,2

1. School of Control Engineering， Northeastern University at Qinhuangdao， Qinhuangdao 066004， China； 2. Hebei Key Laboratory of Micro-nano Precision Optical Sensing and Measurement Technology， Northeastern University at Qinhuangdao， Qinhuangdao 066004， China.

Published:2023-06-20
Contact: HU Sheng
About author:-
Supported by:
-

摘要/Abstract

摘要： 由于红细胞具有双凹形结构和较好的超弹性响应，其能通过大尺度的拉伸和收缩变形穿梭于细长的毛细血管，为维持人体生命活动所需的氧气承担了重要输运工作.本文采用COMSOL有限元软件，依托流-固耦合模块进行了红细胞在不同粗细血管、血浆黏度，以及血液流速等关键影响因素的动态模拟.通过仿真计算可知红细胞能够轻松通过3 μm孔径的毛细血管，相比6 μm孔径的毛细血管承受了4.5倍的流体剪切力.同时5.5 mPa·s黏度的血浆诱使红细胞形成降落伞形状，并且中间凹陷结构消失逐渐沿着流速方向水平凸出.本文对T型和Y型微通道的流速进行了研究，两者的仿真结果都指出非对称流体环境使红细胞形成镰刀弯钩形状.另外，较高流速的支路通道在汇聚口较快诱发红细胞弯钩形状消失，逐渐演变为呈直杆形平躺流动.

关键词: 血管芯片;红细胞;流体动力学;超弹性材料;COMSOL

Abstract: Red blood cell (RBC)， undertaking the significant work of oxygen transport to maintain human life， could shuttle spindly capillaries by their own stretching and contractile properties due to their biconcave discoid shape and hyperelastic responses. In this paper， the coupled fluid-solid module in finite element software COMSOL was used to study the dynamic simulation of RBC when the three factors， including capillary width， plasma viscosity， and blood flow rate were considered. The results implied that the RBC could easily pass through capillary with hole size of 3 μm， and the aqueous shear stress was 4.5 times greater than that in the capillary with 6 μm hole size. Meanwhile， the plasma viscosity of 5.5 mPa·s induced parachute-like formation of RBC. Furthermore， biconcave structure in the center gradually disappeared and turned into bulge along the flow direction. With regard to the study on fluidic velocities in T-shaped and Y-shaped micro-channel， both results indicated that the asymmetric structure caused the falcate shape of RBC. In addition， the higher fluidic velocity in the other branch channel， the easier the convergence leaded to the disappearance of falcate shape and the rod posture lying flat flowing in the solution.

Key words: vessel-on-chip; red blood cell (RBC); hydrodynamics; hyperelastic material; COMSOL

中图分类号:

TN492， TQ460.1

胡晟，叶俊彦，赵勇. 面向血管芯片微通道的红细胞形变学研究[J]. 东北大学学报（自然科学版）, 2023, 44(6): 906-912.

HU Sheng， YE Jun-yan， ZHAO Yong. Study on Morphology of Red Blood Cell in Micro-channel Towards Vessel-on-Chip[J]. Journal of Northeastern University(Natural Science), 2023, 44(6): 906-912.

参考文献

[1]Czerwinska J，Wolf S M，Mohammadi H，et al.Red blood cell aging during storage，studied using optical tweezers experiment［J］.Cellular and Molecular Bioengineering，2015，8(2):258-266.
[2]Mignon T，Mendez S A.Theoretical investigation of the frisbee motion of red blood cells in shear flow［J］.Mathematical Modelling of Natural Phenomena，2021，16:1-28.
[3]戚晓菁，李学进.微流控芯片技术在血细胞变形和流动性分析研究中的应用进展［J］.实验流体力学，2020，34(2):1-10.(Qi Xiao-jing，Li Xue-jin.Research progress on mechanical and flow properties of blood cells in microcirculation using microfluidic devices［J］.Journal of Experiments in Fluid Mechanics，2020，34(2):1-10.)
[4]Ni A，Cheema T A，Kwak M K，et al.Two-dimensional numerical simulation of the red blood cell floating in a plasma-alcohol solution through stenosis in a microvessel［J］.The Korea-Australia Rheology Journal，2014，26(3):293-301.
[5]Ni A，Cheema T A，Park C W.Numerical investigation on the structural characteristics of multiple RBCs in a stenotic microcapillary under plasma-alcohol solution［J］.The Korea-Australia Rheology Journal，2015，27(2):163-171.
[6]Ye H L，Shen Z Q，Li Y.Shape-dependent transport of microparticles in blood flow:from margination to adhesion［J］.Journal of Engineering Mechanics，2019，145(4):1-18.
[7]Cetin A，Sahin M.A monolithic fluid-structure interaction framework applied to red blood cells［J］.International Journal for Numerical Methods in Biomedical Engineering，2019，35(2):e3171.1-e3171.24.
[8]Taraconat P，Gineys J P，Isebe D，et al.Numerical simulation of deformable particles in a coulter counter［J］.International Journal for Numerical Methods in Biomedical Engineering，2019，35(11):1-22.
[9]Jiao Y Y，He Y，Jiao F.Two-dimensional simulation of motion of red blood cells with deterministic lateral displacement devices［J］.Micromachines，2019，10(6):1-15.
[10]Liu Z X，Liu H C，Huang D M，et al.The immersed Boundary-lattice Boltzmann method parallel model for fluid-structure interaction on heterogeneous platforms［J］.Mathematical Problems in Engineering，2020，2020:1-13.
[11]张朦，孟晓辰，祝连庆.基于动态显微成像的红细胞多角度形态学分析方法［J］.光学技术，2021，47(4):417-421.(Zhang Meng，Meng Xiao-chen，Zhu Lian-qing.Multi-angle morphological analysis of red blood cells based on dynamic microscopic imaging［J］.Optical Technique，2021，47(4):417-421.)
[12]Wang Y，Sang J，Ao R，et al.Numerical simulation of deformed red blood cell by utilizing neural network approach and finite element analysis［J］.Computer Methods in Biomechanics and Biomedical Engineering，2020，23(15):1190-1200.
[13]Evans E，Fung Y C.Improved measurements of the erythrocyte geometry［J］.Microvascular Research，1972，4(4):335-347.
[14]王带领，谭建平，程立志，等.基于液固耦合的光镊拉伸红细胞变形特性［J］.中国医学物理学杂志，2017，34(4):404-409.(Wang Dai-ling，Tan Jian-ping，Cheng Li-zhi，et al.Deformation of red blood cells stretching by optical tweezers based on fluid-structure interaction［J］.Chinese Journal of Medical Physics，2017，34(4):404-409.)(上接第905页)
[23]周爱民，余粤.基于流动性囤积视角的银行间流动性风险研究——以2013年“钱荒”为例［J］.金融论坛，2019，24(10):15-23.(Zhou Ai-min，Yu Yue.A research on interbank liquidity risk from the perspective of liquidity hoarding—a case study of “Money Shortage” in 2013［J］.Finance Forum，2019，24(10):15-23.)
[24]苑莹，王海英，庄新田.基于非线性相依的市场间金融传染度量——测度2015年中国股灾对重要经济体的传染效应［J］.系统工程理论与实践，2020，40(3):545-558.(Yuan Ying，Wang Hai-ying，Zhuang Xin-tian.Financial contagion measurement between nonlinear inter-dependent markets: detecting the contagion effects of Chinese stock market crash in 2015 on the world’s important economies［J］.Systems Engineering — Theory & Practice，2020，40(3):545-558.)
[25]Li Y，Zhuang X，Wang J，et al.Analysis of the impact of Sino-US trade friction on China’s stock market based on complex networks［J］.The North American Journal of Economics and Finance，2020，52:101185.
[26]Li Z，Zhou Q，Chen M，et al.The impact of COVID-19 on industry-related characteristics and risk contagion［J］.Finance Research Letters，2021，39: 101931.

面向血管芯片微通道的红细胞形变学研究

Study on Morphology of Red Blood Cell in Micro-channel Towards Vessel-on-Chip

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价