东北大学学报（自然科学版）

东北大学学报(自然科学版) ›› 2010, Vol. 31 ›› Issue (2): 228-231.DOI: -

• 论著 • 上一篇    下一篇

浸矿细菌胞外聚合层中多糖的提取

富瑶;杨洪英;佟琳琳;张长征;   

  1. 东北大学材料与冶金学院;
  • 收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2010-02-15 发布日期:2013-06-20
  • 通讯作者: -
  • 作者简介:-
  • 基金资助:
    国家自然科学基金资助项目(50674029,50874030);;

Polysaccharide extraction from extracellular polymeric substance via bioleaching

Fu, Yao (1); Yang, Hong-Ying (1); Tong, Lin-Lin (1); Zhang, Chang-Zheng (1)   

  1. (1) School of Materials and Metallurgy, Northeastern University, Shenyang 110004, China
  • Received:2013-06-20 Revised:2013-06-20 Online:2010-02-15 Published:2013-06-20
  • Contact: Yang, H.-Y.
  • About author:-
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摘要： 采用EDTA和H2SO4两种溶液作为提取剂,通过离心收集菌体、提取剂浸提多糖、离心分离多糖和过滤去除菌体4个步骤,提取浸矿细菌胞外聚合层中的多糖,并用苯酚-硫酸法测定提取出的多糖的含量.实验结果表明:质量分数相同时,提取剂EDTA的提取效果优于H2SO4;质量分数为3%的EDTA溶液为实验中的最优提取剂,提取的多糖质量浓度可达17.0 mg.L-1.整个提取过程具有使用试剂简单、操作简便、干扰因素少的特点.

关键词: 胞外聚合层, 生物冶金, 浸矿细菌, 多糖

Abstract: With the EDTA (ethylene diamine tetraacetic acid) and H2SO4 (sulfuric acid) solutions both used as the extractants to extract the polysaccharide from EPS (extracellular polymeric substance) via bioleaching, the extracting process includes four steps, i.e., collecting thallus by centrifugation, leaching polysaccharide with extractants, separating polysaccharide by centrifugation and filtering out thallus. The extracted polysaccharide content was measured by phenol-sulfuric acid. The results showed that the extractant EDTA is superior to H2SO4 in extraction effect if their mass fractions are the same, and the 3% EDTA is regarded as the best one in our tests, by which the content of extracted polysaccharide can be up to 0.017 mg·mL-1. The whole extracting process has the advantages of simple reagent required, easy to operate and less interfering factors.

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