东北大学学报（自然科学版）

东北大学学报(自然科学版) ›› 2010, Vol. 31 ›› Issue (2): 273-276.DOI: -

• 论著 • 上一篇    下一篇

光合细菌对对氯苯胺的耐受性研究

李亮;胡筱敏;董怡华;郑玲子;   

  1. 东北大学资源与土木工程学院;
  • 收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2010-02-15 发布日期:2013-06-20
  • 通讯作者: -
  • 作者简介:-
  • 基金资助:
    国家水体污染控制与治理科技重大专项(2008ZX07208-009)

On tolerability of photosynthetic bacteria for p-chloroaniline

Li, Liang (1); Hu, Xiao-Min (1); Dong, Yi-Hua (1); Zheng, Ling-Zi (1)   

  1. (1) School of Resources and Civil Engineering, Northeastern University, Shenyang 110004, China
  • Received:2013-06-20 Revised:2013-06-20 Online:2010-02-15 Published:2013-06-20
  • Contact: Hu, X.-M.
  • About author:-
  • Supported by:
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摘要： 从本溪钢铁焦化厂排污口附近水体浅层底泥中筛选出一株高效降解对氯苯胺(PCA)的紫色非硫光合细菌CS2,实验考察了不同质量浓度PCA对CS2(出发菌株和驯化菌株)生长及其脱氢酶活性的影响.结果表明:随着PCA质量浓度的提高,CS2出发菌的生长和脱氢酶活性会受到明显抑制.CS2驯化后,CS2菌对PCA的耐受性显著提高.采用化学品对水生生物急性毒性试验的标准方法研究了PCA对CS2菌的毒性效应.结果表明,PCA对出发菌株生长的安全质量浓度为60 mg.L-1,抑制CS2菌生长的半抑制浓度96h-IC50为162.5 mg.L-1,驯化后,菌株的安全质量浓度为100 mg.L-1,96h-IC50为4...

关键词: 光合细菌, 对氯苯胺(PCA), 耐受性, 脱氢酶活性, 半抑制浓度

Abstract: A strain of purple non-sulfur bacteria (PNSB) named CS2 was screened from the sediment near the sewage outfall in Benxi Steel Coking Plant. As a photosynthetic strain, CS2 can degrade the p-chloroaniline (PCA) with high efficiency. The effects of concentration of PCA on cell growth and dehydrogenase activity of original/domesticated CS2 strains were investigated. Experimental results showed that with the increasing PCA concentration the growth and dehydrogenase activity of original bacteria are obviously inhabited. However, the tolerability of domesticated CS2 strain for PCA is dramatically improved. The results of a standard method of aquatic bioassay for the toxicity evaluation of chemicals applied in the experiments showed that the lowest observed effects of concentration (LOEC) of the original/domesticated CS2 strain growth are 60 mg/L and 100 mg/L, respectively. On the contrary, the half-inhibitory concentration (IC50) of PCA for the inhibition of original/domesticated CS2 strain growth are 162.5 mg/L and 423.9 mg/L, respectively, i.e., higher than many kinds of bacteria as reported previously.

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