东北大学学报（自然科学版）

东北大学学报(自然科学版) ›› 2011, Vol. 32 ›› Issue (10): 1517-1520.DOI: -

• 论著 • 上一篇    

鸡胸腺素β_4串联体的原核表达、纯化与活性

王国卿;郭弄潮;刘丽;   

  1. 东北大学理学院;黑龙江科技职业学院;
  • 收稿日期:2013-06-19 修回日期:2013-06-19 发布日期:2013-04-04
  • 通讯作者: -
  • 作者简介:-
  • 基金资助:
    中国博士后科学基金资助项目(20080440146;200902492)

Prokaryotic expression, purification and bioactivity of chicken thymosin β4 concatemer

Wang, Guo-Qing (1); Guo, Nong-Chao (1); Liu, Li (2)   

  1. (1) School of Sciences, Northeastern University, Shenyang 110819, China; (2) Heilongjiang Technology Professional College, Shuangcheng 150100, China
  • Received:2013-06-19 Revised:2013-06-19 Published:2013-04-04
  • Contact: Wang, G.-Q.
  • About author:-
  • Supported by:
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摘要： 构建鸡Tβ4基因串联体(2Tβ4)原核表达体系,利用Ni-NTA纯化重组2Tβ4,MTT法检测其免疫活性.结果表明,构建的PET-32a(+)-2Tβ4表达载体在BL21(DE3)感受态细胞中,经0.05 mmol/LIPTG诱导表达,每克菌体平均可获6.7mg 2Tβ4.2Tβ4对淋巴细胞增殖能力与Tβ4标准品相比无显著差异,证明了所构建的鸡2Tβ4原核表达系统的有效性,为Tβ4的应用提供了基础材料.

关键词: 鸡, 胸腺素β4, 串联体, 原核表达, 纯化, 免疫活性

Abstract: The Tβ4 concatemer (2Tβ4) was synthesized by constructing 2Tβ4 prokaryotic expression system. 2Tβ4 was purified and recombined using Ni-NTA spin column, and its immunocompetence was measured by MTT. Under the induction of 0.05 mmol/L IPTG, 6.7 mg 2Tβ4 per gram bacteria was obtained by expressing PET-32a(+)-2Tβ4 in competent cell BL21 (DE3). No significant differences were observed between the effects of 2Tβ4 and standard Tβ4 on the multiplication of lymphocyte. This proves the validity of the established chicken 2Tβ4 prokaryotic expression system, and provides basic material for the application of Tβ4.

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