木茼蒿SOC1同源基因的克隆及表达分析

doi:10.12068/j.issn.1005-3026.2021.04.021

摘要/Abstract

摘要： SOC1基因是整合各种成花途径信号，启动植物成花转变的关键基因之一.以木茼蒿为材料，利用RT-PCR技术，克隆了木茼蒿SOC1同源基因AfSOC1;利用qRT_PCR技术，对其时空表达模式进行了分析.结果表明:木茼蒿AfSOC1 cDNA长为648bp，编码216个氨基酸，与拟南芥SOC1和金鱼草DEFH68的同源性分别为63.1%和62.1%.该蛋白含有典型的MADS-box，K-box和SOC1转录因子特异性识别序列DVETELFIGP.AfSOC1在木茼蒿的根、茎、叶、花芽及花中均有表达，在叶中和花芽中高表达.在系统进化树中AfSOC1与所有菊科植物SOC1聚在一起.该研究为了解木茼蒿的成花机制及通过分子生物学技术进行木茼蒿的遗传改良提供了理论基础.

关键词: 木茼蒿;SOC1同源基因;实时定量PCR，克隆，分子系统

Abstract: SOC1 is one of crucial genes in integrating several pathway signals to initiate the transition of flowering. The SOC1 homologue gene AfSOC1 was identified and cloned from Argyranthemum frutescens using RT-PCR and its expression patterns were characterized using qRT-PCR. The results show that AfSOC1 gene consists of a 648-bp open reading frame and encodes a putative protein of 216 amino acids， which is 63.1% identical to SOC1 in Arabidopsis and 62.1% to DEFH68 in Antirrhinum. The putative protein sequences have a typical MADS-box， K-box domain and typical SOC1 motif DVETELFIGP. AfSOC1 was expressed at highly levels in leaves and flower bud. Its expression signals were also detected in shoot， stems， and flowers. Phylogenetic analysis showed that AfSOC1 was most closely related to dicotyledon SOC1-like than to monocotyledon. These results laid a foundation for us to understand the mechanism of AfSOC1 in regulation flowering.

Key words: Argyranthemum frutescens; SOC1 homologue gene; qRT-PCR; cloning， molecular phylogeny

中图分类号:

TP20

马月萍，胡静. 木茼蒿SOC1同源基因的克隆及表达分析[J]. 东北大学学报（自然科学版）, 2021, 42(4): 604-608.

MA Yue-ping， HU Jing. Cloning and Expression Analysis of SOC1 Homologue Gene in Argyranthemum Frutescens[J]. Journal of Northeastern University(Natural Science), 2021, 42(4): 604-608.

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