煤矸石及其自燃风化土中可培养细菌的分离与解磷抗镉特性

doi:10.12068/j.issn.1005-3026.2020.10.012

东北大学学报:自然科学版 ›› 2020, Vol. 41 ›› Issue (10): 1438-1444.DOI: 10.12068/j.issn.1005-3026.2020.10.012

煤矸石及其自燃风化土中可培养细菌的分离与解磷抗镉特性

姜彬慧，姜琦，周星星，施洋

(东北大学资源与土木工程学院，辽宁沈阳110819)

收稿日期:2020-03-09 修回日期:2020-03-09 出版日期:2020-10-15 发布日期:2020-10-20
通讯作者: 姜彬慧
作者简介:姜彬慧(1962-)，女，辽宁沈阳人，东北大学教授.
基金资助:
国家重点研发计划项目(2017YFC1503105).

Isolation of Cultivable Bacteria in Coal Gangue Spontaneous Combustion Weathered Soil and Phosphate-Solubilizing and Cadmium-Resistant Characteristics

JIANG Bin-hui， JIANG Qi， ZHOU Xing-xing， SHI Yang

School of Resources & Civil Engineering， Northeastern University， Shenyang 110819， China.

Received:2020-03-09 Revised:2020-03-09 Online:2020-10-15 Published:2020-10-20
Contact: JIANG Qi
About author:-
Supported by:
-

摘要/Abstract

摘要： 采用平板涂布分离法，从抚顺西露天煤矿内排土场煤矸石风化土(MF)及煤矸石自燃风化土(MZ)中分离纯化出3株细菌，标记为MF2，MZ1和MZ2.利用16S rRNA基因序列检测，结合细菌形态与生理生化特征对其进行种属鉴定与分类，其中菌株MF2为类节杆菌属(Paenarthrobacter sp.)细菌，MZ1为抗辐射不动杆菌(Acinetobacter radioresistens)，MZ2为拟蕈状芽孢杆菌(Bacillus paramycoides).分别考察了菌株的解磷能力和对重金属Cd²⁺的耐受性.结果发现:3株细菌的最大解磷能力依次为MF2(72h，71.76mg·L^-1)>MZ2(96h，55.48mg·L^-1)>MZ1(120h，5.79mg·L^-1)，对Cd²⁺的最大耐受剂量排序为MZ2(100mg·L^-1)>MZ1(50mg·L^-1)>MF2(10mg·L^-1)，其中拟蕈状芽孢杆菌MZ2既具有较强的解磷能力，同时对重金属Cd²⁺又有较高的耐受性.

关键词: 煤矸石自燃风化土, 镉污染土壤, 可培养微生物, 解磷抗镉菌, 生物磷肥

Abstract: Using plate coating separation method， 3 strains of bacteria were isolated and purified from coal gangue weathered soil (MF) and coal gangue spontaneously weathered soil (MZ) in Fushun West Open-pit Coal Mine， labeled as MF2， MZ1 and MZ2. Using 16S rRNA gene sequence detection， the strains of bacteria were species identified and classified according to bacterial morphology and physiological and biochemical characteristics， of which strain MF2 is Paenarthrobacter sp.， MZ1 is Acinetobacter radioresistens， MZ2 is Bacillus paramycoides. The strains’ abilities of phosphate-solubilizing and cadmium tolerance were respectively investigated. The results showed that the maximum phosphate-solubilizing capacity of the three bacteria is MF2 (72h， 71.76mg·L^-1)> MZ2 (96h， 55.48mg·L^-1)> MZ1 (120h， 5.79mg·L^-1)， and the maximum tolerated dose of Cd²⁺ is ranked as MZ2 (100mg·L^-1)>MZ1 (50mg·L^-1)>MF2 (10mg·L^-1). The B. paramycoides MZ2 not only has strong ability to dissolve phosphorus， but also has high tolerance to heavy metal Cd²⁺ among three strains.

Key words: coal gangue spontaneous combustion weathered soil, cadmium pollution soil, culturable microorganisms, phosphate-solubilizing and cadmium-resistant bacteria, biological phosphate fertilizer

中图分类号:

姜彬慧，姜琦，周星星，施洋. 煤矸石及其自燃风化土中可培养细菌的分离与解磷抗镉特性[J]. 东北大学学报:自然科学版, 2020, 41(10): 1438-1444.

JIANG Bin-hui， JIANG Qi， ZHOU Xing-xing， SHI Yang. Isolation of Cultivable Bacteria in Coal Gangue Spontaneous Combustion Weathered Soil and Phosphate-Solubilizing and Cadmium-Resistant Characteristics[J]. Journal of Northeastern University Natural Science, 2020, 41(10): 1438-1444.

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煤矸石及其自燃风化土中可培养细菌的分离与解磷抗镉特性

Isolation of Cultivable Bacteria in Coal Gangue Spontaneous Combustion Weathered Soil and Phosphate-Solubilizing and Cadmium-Resistant Characteristics

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