东北大学学报（自然科学版）

东北大学学报(自然科学版) ›› 2006, Vol. 27 ›› Issue (4): 363-366.DOI: -

• 论著 • 上一篇    下一篇

温度对诺西肽发酵过程影响的建模与仿真

张大鹏;王福利;何建勇;何大阔;   

  1. 东北大学信息科学与工程学院;东北大学信息科学与工程学院;沈阳药科大学制药工程学院;东北大学信息科学与工程学院 辽宁沈阳110004;辽宁沈阳110004;辽宁沈阳110004;辽宁沈阳110004
  • 收稿日期:2013-06-23 修回日期:2013-06-23 出版日期:2006-04-15 发布日期:2013-06-23
  • 通讯作者: Zhang, D.-P.
  • 作者简介:-
  • 基金资助:
    国家自然科学基金资助项目(60374003);;

Modeling and simulation of influence of temperature on batch fermentation process of nosiheptide

Zhang, Da-Peng (1); Wang, Fu-Li (1); He, Jian-Yong (2); He, Da-Kuo (1)   

  1. (1) School of Information Science and Engineering, Northeastern University, Shenyang 110004, China; (2) School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2013-06-23 Revised:2013-06-23 Online:2006-04-15 Published:2013-06-23
  • Contact: Zhang, D.-P.
  • About author:-
  • Supported by:
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摘要： 研究了温度对诺西肽分批发酵过程的影响.温度对菌体生长率的影响用Arrennius方程表示,对产物生成的影响由实验值拟合得到,对溶解氧的影响通过建立与培养基成分关联的溶解氧模型进行研究,在此基础上对诺西肽原有的恒温恒压分批发酵模型进行了改进,并通过100 L发酵罐对改进模型进行了验证.实验结果表明改进模型能很好地符合实验结果.在改进模型基础上进行仿真研究,结果发现:26~32℃范围内,在指数生长期,菌体生长随温度升高而加快,但不同温度下的最终菌体浓度基本不变;在静止期,菌体平均浓度不因温度不同而发生较大变化;产物28℃时生成最多,温度过高或过低均会导致产量降低.

关键词: 诺西肽, 分批发酵, 比生长率, 溶解氧, 建模, 仿真

Abstract: How the temperature affects the batch fermentation process of Nosiheptide was investigated via modeling and simulation. Where its direct influence on biomass growth rate is expressed by Arrennius equation and that on product yield is given by fitting experimental values. The indirect influence of temperature on dissolved oxygen was investigated via modeling in relation to the ingredients of medium. Then, the model of batch fermentation process of Nosiheptide at constant temperature/pressure was improved, and it was further verified via an experiment done in a 100 L fermenter. It is shown that the improved model conforms well to experimental results. A simulation test based on the improved model was thus conducted and the results showed exponentially that in the range from 26°C to 32°C the biomass growth rate increases with temperature but, eventually, the biomass concentration almost remains unchanged no matter how high/low the temperature is. In stationary phase however the mean biomass concentration changes slightly with changing temperature. The maximum product yield is found at 28°C, which will decrease if the temperature is too high or low.

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