胞外聚合物的提取及其吸附性能

doi:10.12068/j.issn.1005-3026.2021.10.014

摘要/Abstract

摘要： 为了快速、高效地提取活性污泥的胞外聚合物(extracellular polymeric substances，EPS)，采用4种方法提取紧密结合型EPS(tightly bound EPS，TB-EPS)，对比不同提取方法的优缺点，考察TB-EPS对Mn²⁺吸附性能的影响，研究了TB-EPS的吸附机理.结果表明:加热法提取的TB-EPS效率高，方法简单，操作方便，不破坏细胞结构.加热法提取的TB-EPS用于吸附重金属Mn²⁺，其最大吸附率为53.8%，吸附过程符合二级反应动力学特征和Langmuir吸附等温方程，该吸附以化学吸附为主.在TB-EPS中色氨酸和酪氨酸的疏水作用是主要的吸附机理.

关键词: 活性污泥;胞外聚合物(EPS);提取方法;吸附;Mn²⁺

Abstract: In order to quickly and efficiently extract extracellular polymeric substances(EPS)from activated sludge， four methods were used to extract tightly bound EPS(TB-EPS). The advantages and disadvantages of different extraction methods were compared， the effect of TB-EPS on the adsorption performance of Mn²⁺was investigated， and the adsorption mechanism of TB-EPS was studied. The results show that the heating method is simple and efficient with no damage to cell structure.When adsorbing heavy metal Mn²⁺， the maximum adsorption rate of TB-EPS extracted by heating method is 53.8%. The adsorption process conforms to the second-order reaction kinetic characteristics and the Langmuir adsorption isotherm equation， which is mainly the chemical adsorption. The main adsorption mechanism of TB-EPS is the interaction of hydrophobic tryptophan and tyrosin.

Key words: activated sludge; extracellular polymeric substances(EPS); extraction method; adsorption; Mn²⁺

中图分类号:

TP20

徐小惠，魏德洲，张兰河. 胞外聚合物的提取及其吸附性能[J]. 东北大学学报（自然科学版）, 2021, 42(10): 1467-1474.

XU Xiao-hui， WEI De-zhou， ZHANG Lan-he. Extraction and Adsorption Properties of Extracellular Polymeric Substance[J]. Journal of Northeastern University(Natural Science), 2021, 42(10): 1467-1474.

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