Isolation of Cultivable Bacteria in Coal Gangue Spontaneous Combustion Weathered Soil and Phosphate-Solubilizing and Cadmium-Resistant Characteristics

doi:10.12068/j.issn.1005-3026.2020.10.012

Abstract

Abstract: Using plate coating separation method， 3 strains of bacteria were isolated and purified from coal gangue weathered soil (MF) and coal gangue spontaneously weathered soil (MZ) in Fushun West Open-pit Coal Mine， labeled as MF2， MZ1 and MZ2. Using 16S rRNA gene sequence detection， the strains of bacteria were species identified and classified according to bacterial morphology and physiological and biochemical characteristics， of which strain MF2 is Paenarthrobacter sp.， MZ1 is Acinetobacter radioresistens， MZ2 is Bacillus paramycoides. The strains’ abilities of phosphate-solubilizing and cadmium tolerance were respectively investigated. The results showed that the maximum phosphate-solubilizing capacity of the three bacteria is MF2 (72h， 71.76mg·L^-1)> MZ2 (96h， 55.48mg·L^-1)> MZ1 (120h， 5.79mg·L^-1)， and the maximum tolerated dose of Cd²⁺ is ranked as MZ2 (100mg·L^-1)>MZ1 (50mg·L^-1)>MF2 (10mg·L^-1). The B. paramycoides MZ2 not only has strong ability to dissolve phosphorus， but also has high tolerance to heavy metal Cd²⁺ among three strains.

Key words: coal gangue spontaneous combustion weathered soil, cadmium pollution soil, culturable microorganisms, phosphate-solubilizing and cadmium-resistant bacteria, biological phosphate fertilizer

CLC Number:

JIANG Bin-hui， JIANG Qi， ZHOU Xing-xing， SHI Yang. Isolation of Cultivable Bacteria in Coal Gangue Spontaneous Combustion Weathered Soil and Phosphate-Solubilizing and Cadmium-Resistant Characteristics[J]. Journal of Northeastern University Natural Science, 2020, 41(10): 1438-1444.

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