Abstract: Taking the Gluconacetobacter xylinus Gyll as strain, the fermentation conditions for the preparation of bacterial cellulose was optimized by L18(3⁷) orthogonal test, and the bacterial cellulose thus prepared was used for the adsorption of Cd²⁺. Then, the effects of such performance parameters as pH value and adsorbing time on the efficiency of adsorption were investigated, with the adsorption kinetics and adsorption isotherms simulated. The results showed that the optimal ferment conditions for preparing bacterial cellulose are as follows: corn steep powder 5%, sucrose 6%, the initial medium pH value 6.0, fermentation broth inoculum 12%, the second species of seed age for enlargement cultivation 22 hr and static culture period 7 days. The highest yield of bacterial cellulose is 12.36 g/L. In the process with bacterial cellulose as sorbent for removing Cd²⁺, the pH value is a very important parameter to the adsorption efficiency. The adsorption rate could be well fitted by pseudo-second rate model, and the adsorption isotherm could be described by Langmuir model, both having good linearity. All these facts verify that the bacterial cellulose provides a typical monomolecular-layer adsorption of Cd²⁺.