Abstract: The inhibition effects of Cu²⁺ on seed germination and radicle/germ elongation were studied via hydroponical experiment, and the effects of different Cu²⁺ contents in soil on the length, weight and chlorophyll content of above-ground and underground parts of plants were studied via pot culture experiment. The results revealed that the aqueous solution in which the Cu²⁺ mass concentration greater than or equal 400 mg·L^-1 obviously inhibits the seed germination of the three plants: Medicago sativa, Trifolium pratense and Astragalus adsurgens. The higher the Cu²⁺ mass concentration, the more obvious the inhibition effect, and the inhibition effect on the first one is lower than that on the other two. In addition, the inhibition effect on the elongation of radicle is higher than that on germ. Excessive Cu²⁺ content will lower the chlorophyll content in plants with obvious inhibition effect on the growth of above-ground parts of plants. However, the Cu²⁺ content clearly accelerates the growth of length and weight of the root system of M. sativa and T. pratense, but it inhibits that of A. adsurgens obviously. As a whole, the endurability of M. sativa to Cu pollution is higher than that of the other two plants.