Abstract: The RE-doped TiO2 such as i.e. Ce/TiO2, Pr/TiO2, Gd/TiO2, Dy/TiO2, Y/TiO2 and Eu/TiO2, photocatalytic antibacterial films were prepared by sol-gel method in combination with spinning coating process. The effects of sintering schedule, temperature, time and doping amount on their bactericidal potency are investigated to determine the optimum conditions for preparing the six types of RE-doped TiO2 films mentioned above. The results revealed that the structures of all RE/TiO2 films are made up singly of anatase, except for the Ce/TiO2 films which contain few rutile in addition to anatase. However, all of their surfaces are found defective obviously. Comparing with the pure TiO2 films whose average bactericidal rate to E. Coli is 34%, that of all the above six films are over 90% because the RE doping improves greatly the photocatalytic materials in response to visible light. The films have durably high bactericidal rate and stability even after used repeatedly and treated or washed at high temperature and under high pressure without peel-off found, thus providing high performance and good prospects in application.