Abstract: Substances in Stellera chamaejasme root were extracted by water and ethanol separately, and the influences of the two extraction liquids of different concentrations, i.e., diluted to 0, 10, 20 and 50 times as thin as original extraction liquids, on seed germination and physiological indexes of 2 plants were studied. The results showed that both the original extraction liquids and their 10-20 times dilutions significantly inhibit the seed germination percentage and germinating process. The inhibitability of ethanol-extracted liquid is higher than that of water-extracted one, and the lower the concentration, the weaker the inhibitability. Physiological responses of the 2 plants were induced under extraction liquid treatments. It was found the soluble sugar and protein contents in seeds decrease, while proline content in seeds and malondialdehyde (MDA) content in seedlings increase with weakened superoxide dismutase (SOD) activity.