Abstract: Based on literature review and basic assumptions, a model of demand structure was constructed. Questionnaire survey was carried out on the knowledge workers of the industry-university-research knowledge alliance enterprises in the national scope, and the empirical research results indicated that the main demand factors of knowledge workers include explicit demands, tacit demands and active demand. Knowledge worker's sensitivity level of explicit demand factors, tacit demand factors and active demand factors had positive correlation with emerging demand desire. Furthermore, it could be concluded that knowledge workers' individual differences, such as sex, age, education and position, would significantly affect their feel degree of any demand factors.