Abstract: S-11 adhesive was used to paste gold concentrate around low-grade primary ore and the column bioleaching was compared with traditional column bioleaching. The concentration of Fe²⁺, pH, Eh, attached bacteria concentration on the surface of ore, free bacteria concentration in leaching solution, oxidation rates of arsenic and sulfur and cyaniding leaching efficiency were measured. The results show that it is better for bacterial reproduction when a coating of gold concentrate is pasted on the low-grade primary ore. The oxidation rate of arsenic is 70.14%, oxidation rate of sulfur is 41.26%, and cyaniding leaching efficiency is 81.21% after 120 days by column bioleaching. The rates are 24.01%, 19.11% and 21.48% higher than traditional column bioleaching, respectively.