Abstract: A novel synthesis method, called poly(vinyl alcohol) assisted foaming method (PAF method) for the preparation of porous hydroxyapatite (HA) scaffolds with 3D-interconnected macropores without using any templates or porogens or complicated chemical process was reported. XRD analyses revealed that the sintered macroporous HA is pure in compositions. SEM and CLSM results showed that the macroporous structures and pore sizes can be facilely tuned by adjusting the mass ratios of PVA to HA. The mass ratios PVA to HA of 4.4 and 4.9 produce macroporous HAs with uniformly distributed macropores with sizes ranging from 90 to 200 μm in sizes, which are similar to those of human bone in terms of macropore size and interconnectivity. It is therefore believed that such kind of 3D interconnected macroporous HA scaffolds have great potential for application in the area of bone tissue engineering.