基于高通量测序技术的阳极生物膜群落结构

doi:10.12068/j.issn.1005-3026.2016.01.022

东北大学学报:自然科学版 ›› 2016, Vol. 37 ›› Issue (1): 105-109.DOI: 10.12068/j.issn.1005-3026.2016.01.022

基于高通量测序技术的阳极生物膜群落结构

王有昭，朱彤，谢元华，李现瑾

(东北大学机械工程与自动化学院，辽宁沈阳110819)

收稿日期:2014-10-27 修回日期:2014-10-27 出版日期:2016-01-15 发布日期:2016-01-08
通讯作者: 王有昭
作者简介:王有昭(1984-)，男，辽宁大连人，东北大学博士后研究人员；朱彤(1963-)，男，辽宁沈阳人，东北大学教授，博士生导师.
基金资助:
中国博士后科学基金资助项目(2015M570255)；中央高校基本科研业务费专项资金资助项目(N140303002); 辽宁省教育厅科学技术研究项目(L20150178); 东北大学博士后科研基金资助项目(20150304).

Community Structure of Anode Biofilm by High-Throughput Sequencing Technology

WANG You-zhao，ZHU Tong，XIE Yuan-hua，LI Xian-jin

School of Mechanical Engineering & Automation，Northeastern University，Shenyang 110819，China.

Received:2014-10-27 Revised:2014-10-27 Online:2016-01-15 Published:2016-01-08
Contact: ZHU Tong
About author:-
Supported by:
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摘要/Abstract

摘要： 利用高通量测序技术对阳极生物膜的微生物群落结构进行了分析.实验结果表明，高通量测序能够高效地获取电化学生物膜的群落结构信息，产电模式菌Geobacter属在生物膜中占据着优势地位，其数量比例达到30%左右，其次的主要菌属为Thermovirga，Thauera和 Syntrophorhabdus.对比电化学活性丧失前后的生物膜发现，虽然Geobacter菌属在丧失了电化学活性的生物膜中仍然占据统治地位，但是放线菌门的细菌增长显著，其数量比例远高于正常阳极生物膜的0.5%，有可能是抑制生物膜电化学活性的因素之一.

关键词: 阳极生物膜, 生物电化学系统, 高通量测序, 微生物多样性, 16S rRNA

Abstract: Microbial community structure of anode biofilm was analyzed using high-throughput sequencing technology. The results show that the technology can obtain community structure information from electrochemistry biofilm efficiently， and the exoelectrogentic modal bacteria Geobacter category is predominant in biological membrane， its quantity ratio reaches 30%；Thermovirga，Thauera and Syntrophorhabdus are also found in anode biofilm. However， through comparison of biofilm before and after electrochemical activity was lost， Geobacter still dominated in biofilm after the biofilm lost electrochemical activity，while Actinomycetes’ proportion increased significantly， its quantity was much higher than that of the normal 0.5%. So Actinomycetes may be one of the factors which inhibit the electrochemical activity of the biofilm.

Key words: anode biofilm, bioelectrochemical system (BES);high-throughput sequencing, microbial diversity, 16S rRNA

中图分类号:

王有昭，朱彤，谢元华，李现瑾. 基于高通量测序技术的阳极生物膜群落结构[J]. 东北大学学报:自然科学版, 2016, 37(1): 105-109.

WANG You-zhao，ZHU Tong，XIE Yuan-hua，LI Xian-jin. Community Structure of Anode Biofilm by High-Throughput Sequencing Technology[J]. Journal of Northeastern University Natural Science, 2016, 37(1): 105-109.

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基于高通量测序技术的阳极生物膜群落结构

Community Structure of Anode Biofilm by High-Throughput Sequencing Technology

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