PCR-DGGE方法解析电凝聚膜生物反应器中微生物群落结构

doi:10.12068/j.issn.1005-3026.2014.05.033

东北大学学报:自然科学版 ›› 2014, Vol. 35 ›› Issue (5): 751-755.DOI: 10.12068/j.issn.1005-3026.2014.05.033

PCR-DGGE方法解析电凝聚膜生物反应器中微生物群落结构

李亮，胡筱敏，余丹静，陈文希

(东北大学资源与土木工程学院，辽宁沈阳110819)

收稿日期:2013-07-20 修回日期:2013-07-20 出版日期:2014-05-15 发布日期:2014-08-18
通讯作者: 李亮
作者简介:李亮(1980-)，男，江西九江人，东北大学博士研究生;胡筱敏(1958-)，男，江西婺源人，东北大学教授，博士生导师.
基金资助:
国家自然科学基金资助项目(51178088);国家水体污染控制与治理重大专项(2013ZX07202-010).

PCRDGGE Analysis of Microbial Community Structure in a Electrocoagulation Membrane Bioreactor

LI Liang， HU Xiaomin， YU Danjing， CHEN Wenxi

School of Resources & Civil Engineering， Northeastern University， Shenyang 110819， China.

Received:2013-07-20 Revised:2013-07-20 Online:2014-05-15 Published:2014-08-18
Contact: HU Xiaomin
About author:-
Supported by:
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摘要/Abstract

摘要： 在浸没式膜生物反应器(SMBR)膜组件两侧设置以铁为牺牲阳极的电凝聚极板，构建电凝聚膜生物反应器(ECMBR)处理活性艳蓝X-BR模拟印染废水，与传统SMBR相比，ECMBR中活性污泥对COD，NH+4—N，TN，TP和染料的去除率分别提高了27%，38%，47%，392%和286%.应用PCR-DGGE方法，对普通SMBR和ECMBR中的微生物群落结构的变化进行分析.结果表明，运行期ECMBR中污泥的多样性Shannon指数为29，略高于同期的SMBR，两个反应器中的微生物群落结构变化很小，相似性维持在97%以上，在膜生物反应器中采用电凝聚强化微生物多样性是可行的.

关键词: 膜生物反应器, 电凝聚, 微生物多样性, PCR-DGGE, 克隆测序

Abstract: A new submerged electrocoagulation membrane bioreactor (ECMBR) was developed by installing ferric plates on both sides of membrane module in the traditional submerged membrane bioreactor (SMBR) to treat the simulated dying and printing wastewater with active brilliant blue XBR. The removal efficiency of ECMBR on COD， NH+4—N， TN， TP and chroma was respectively 2.7%， 3.8%， 4.7%， 39.2% and 28.6% higher than that of SMBR. The PCRDGGE analysis was used to explore the evolution of bacterial community in ECMBR and SMBR. The diversity Shannon index of the whole bacterial community in ECMBR was 2.9， higher than that in SMBR. Moreover， the bacterial community structure underwent small changes， with a 97% similarity in both ECMBR and SMB. The results showed that it was possible to enhance the bacterial community diversity by electrocoagulation.

Key words: membrane bioreactor (MBR), electrocoagulation, micro diversity, PCRDGGE, cloned sequencing

中图分类号:

X172

李亮，胡筱敏，余丹静，陈文希. PCR-DGGE方法解析电凝聚膜生物反应器中微生物群落结构[J]. 东北大学学报:自然科学版, 2014, 35(5): 751-755.

LI Liang， HU Xiaomin， YU Danjing， CHEN Wenxi. PCRDGGE Analysis of Microbial Community Structure in a Electrocoagulation Membrane Bioreactor[J]. Journal of Northeastern University Natural Science, 2014, 35(5): 751-755.

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PCR-DGGE方法解析电凝聚膜生物反应器中微生物群落结构

PCRDGGE Analysis of Microbial Community Structure in a Electrocoagulation Membrane Bioreactor

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