东北大学学报（自然科学版）

东北大学学报(自然科学版) ›› 2013, Vol. 34 ›› Issue (3): 444-447.DOI: -

• 生物工程 • 上一篇    下一篇

外周血淋巴细胞中端粒酶活性激活及机制的研究

雷红玮，田亚男，王旭   

  1. (东北大学信息科学与工程学院，辽宁沈阳110819)
  • 收稿日期:2012-06-04 修回日期:2012-06-04 出版日期:2013-03-15 发布日期:2013-01-26
  • 通讯作者: 雷红玮
  • 作者简介:雷红玮(1975-)，女，河南安阳人，东北大学博士研究生；王旭(1956-)，男，辽宁沈阳人，东北大学教授，博士生导师.
  • 基金资助:
    国家自然科学基金资助项目(60970157).

Research of Telomerase Activity in Lymphocytes of Human Peripheral Blood

LEI Hongwei， TIAN Yanan， WANG Xu   

  1. School of Information Science ＆Engineering， Northeastern University， Shenyang 110819， China.
  • Received:2012-06-04 Revised:2012-06-04 Online:2013-03-15 Published:2013-01-26
  • Contact: LEI Hongwei
  • About author:-
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摘要： 实验采用PHA和rhIL-2激活人外周血淋巴细胞，再以流式细胞术(FCM)分析细胞周期和细胞分裂增殖；以TRAP检测外周血淋巴细胞活化前后端粒酶活性的变化；以Westernblot检测hTERT和相关蛋白的表达；以荧光定量PCR分析hTERT的mRNA变化水平.结果表明:外周血淋巴细胞在被PHA和rhIL-2刺激活化后，端粒酶活性升高，升高的端粒酶活性能够被JAK抑制剂所抑制，提示端粒酶活性的升高依赖JAK信号通路.升高的端粒酶活性是由于hTERT蛋白表达的增高，而hTERT表达增高的原因是由于hTERT的mRNA表达水平升高，实验还表明这些活动都同样依赖于JAK信号通路.本研究深化了对端粒酶活性以及hTERT调控机制的认识.

关键词: 端粒酶活性, 淋巴细胞, hTERT, mRNA, JAK信号通路

Abstract: PHA and rhIL2 were adopted to stimulate human peripheral blood lymphocytes. The cell cycle and cell proliferation were analyzed based on the flowing cytometry (FCM). By using TRAP， the changes of telomerase activity in peripheral blood lymphocytes before and after activation were detected. By using Western blot， the expression of hTERT and related protein were detected. By using the fluorescent quantitative PCR， the mRNA levels of hTERT were analyzed. The results showed that after the peripheral blood lymphocytes were activated by PHA and rhIL2， telomerase activity was elevated， and elevated telomerase activity could be restrained by the JAK inhibitor. These results indicated that the increase of the telomerase activity depends on JAK signal pathway. Elevated telomerase activity is due to the increase of hTERT protein expression， while the hTERT is due to the increase of mRNA expression levels of hTERT. The results of the experiments showed that these activities also depends on the JAK signal pathway. Evidences about the telomerase activity and hTERT regulation mechanism are provided based on this study.

Key words: telomerase activity, lymphocyte, hTERT, mRNA, JAK signal pathway

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