一株嗜酸氧化亚铁硫杆菌的鉴定及浸矿性能

doi:10.12068/j.issn.1005-3026.2016.01.019

东北大学学报:自然科学版 ›› 2016, Vol. 37 ›› Issue (1): 89-93.DOI: 10.12068/j.issn.1005-3026.2016.01.019

一株嗜酸氧化亚铁硫杆菌的鉴定及浸矿性能

卢涛¹，魏德洲¹，张瑞洋¹，卓建英²

(1. 东北大学资源与土木工程学院，辽宁沈阳110819； 2. 辽宁省冶金地质勘查局四〇三队，辽宁鞍山114018)

收稿日期:2014-11-10 修回日期:2014-11-10 出版日期:2016-01-15 发布日期:2016-01-08
通讯作者: 卢涛
作者简介:卢涛(1986-)，男，山东淄博人，东北大学博士研究生；魏德洲(1956-)，男，河南南阳人，东北大学教授，博士生导师.
基金资助:
中央高校基本科研业务费专项资金资助项目(N120601005); “十二五”国家科技支撑计划项目(2012BAB01B03).

Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan

LU Tao¹， WEI De-zhou¹， ZHANG Rui-yang¹， ZHUO Jian-ying²

1.School of Resources & Civil Engineering， Northeastern University， Shenyang 110819，China; 2. Team 403 in Liaoning Provincial Bureau of Metallurgical & Geological Prospecting， Anshan 114018， China.

Received:2014-11-10 Revised:2014-11-10 Online:2016-01-15 Published:2016-01-08
Contact: LU Tao
About author:-
Supported by:
-

摘要/Abstract

摘要： 从西藏甲玛某多金属矿的酸性矿坑水中分离获得一株浸矿细菌(命名为XZ).该菌为革兰氏阴性菌，短杆状，菌体长约1～1.5μm，直径约0.5μm，两端钝圆，能够氧化Fe²⁺和单质硫，最适生长pH为2.0，最适生长温度为30℃，经过对其生长特性进行研究及16S rRNA基因测序鉴定其为一株嗜酸氧化亚铁硫杆菌.利用XZ菌对西藏某低品位铜矿角岩矿进行了摇瓶浸出试验研究，结果表明，在接种量体积分数为10%，矿浆质量分数为10%，矿石粒度为-45μm占100%，培养温度30℃，振荡强度160r/min的浸出条件下，经过15d，Cu²⁺的浸出率达72.15%.

关键词: 嗜酸氧化亚铁硫杆菌, 菌种鉴定, 16S rRNA, 生物浸出, 低品位铜矿

Abstract: A bioleaching bacterium was isolated from an acidic mine drainage of Jiama polymetallic mine in Tibet and was named as XZ. The strain XZ， with round ends， is gram-negative and short-rod shaped in 1～1.5μm×0.5μm. Ferrous iron and elemental sulfur could be oxidized by strain XZ. The optimum growth pH is 2.0 and the optimum growth temperature is 30℃. It was identified as Acidithiobacillus ferrooxidans by means of growth characteristic research and 16S rRNA sequencing. The strain XZ was used to leach a poor copper hornstone ore took from Tibet.Shaking flask leaching tests were carried out with inoculation size of 10%(volume fraction)， pulp mass fraction of 10% ，ore particle size of -45μm accounting for 100%， leaching temperature of 30℃ and shaking intensity of 160r/min. The results show that leaching rate of copper can reach 72.15% after 15 days.

Key words: Acidithiobacillus ferrooxidans, strain identification, 16S rRNA, bioleaching, poor copper ore

中图分类号:

TD925.5
Q939

卢涛，魏德洲，张瑞洋，卓建英. 一株嗜酸氧化亚铁硫杆菌的鉴定及浸矿性能[J]. 东北大学学报:自然科学版, 2016, 37(1): 89-93.

LU Tao， WEI De-zhou， ZHANG Rui-yang， ZHUO Jian-ying. Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan[J]. Journal of Northeastern University Natural Science, 2016, 37(1): 89-93.

参考文献

[1]Brierley J A，Brierley C L.Present and future commercial applications of biohydrometallurgy［J］.Hydrometallurgy，2001，59:233-239.
[2]魏德洲，朱一民，李晓安.生物技术在矿物加工中的应用［M］.北京:冶金工业出版社，2008:56-57.(Wei De-zhou，Zhu Yi-min，Li Xiao-an.Application of biotechnology in minerals engineering［M］.Beijing:Metallurgical Industry Press，2008:56-57.)
[3]Yang S R，Xie J Y，Qiu G Z，et al.Research and application of bioleaching and biooxidation technologies in China［J］.Minerals Engineering，2002，15:361-363.
[4]Kelly D P，Wood A P.Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen.nov.，Halothiobacillus gen.nov.and Thermithiobacillus gen.nov.［J］.International Journal of Systematic and Evolutionary Microbiology，2000，50:511-516.
[5]Olson G J，Brierley J A，Brierley C L.Bioleaching review part B.progress in bioleaching:applications of microbial processes by the minerals industries［J］.Applied Microbiology Biotechnology，2003，63:249-257.
[6]张在海，邱冠周，胡岳华，等.氧化亚铁硫杆菌的菌落分离研究［J］.矿产综合利用，2001(1):19-23.(Zhang Zai-hai，Qiu Guan-zhou，Hu Yue-hua，et al.The investigation of the colony isolation of Thiobacillus ferrooxidans［J］.Multipurpose Utilization of Mineral Resources，2001(1):19-23.
[7]Peng H，Yang Y，Li X，et al.Sturcture analysis of 16S rRNA sequences of strains from Acidithiobacillus ferrooxidans［J］.Journal of Biochemistry and Molecular Biology，2006，39(2):178-182.
[8]杨宇，万民熙，彭宏，等.一株黄铜矿专属浸出细菌的分离与鉴定［J］.中南大学学报(自然科学版)，2007，38(4):639-645.(Yang Yu，Wan Min-xi，Peng Hong，et al.Isolation and characterization of bacterium for chalcopyrite bioleaching［J］.Journal of Central South University(Natural Science)，2007，38(4):639-645.
[9]Rawlings D E.Characteristics and adaptability of iron- and sulfur-oxidizing microorganisms used for the recovery of metals from minerals and their concentrates［J］.Microbial Cell Factories，2005，4:13.
[10]Xia J L，Peng A A，He H，et al.A new strain Acidithiobacillus albertensis BY-05 for bioleaching of metal sulfides ores［J］.Transactions of Nonferrous Metal Society of China，2007，17(1):168-175.(上接第83页)

一株嗜酸氧化亚铁硫杆菌的鉴定及浸矿性能

Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 9

编辑推荐

Metrics

本文评价

[1]	姜良友，魏德洲，刘凯凯，张浩. 基于不同粒级硫化铜矿石生物浸出的动力学[J]. 东北大学学报:自然科学版, 2019, 40(10): 1491-1495.
[2]	张瑞洋，魏德洲，刘文刚，高淑玲. Triton X-100对氧化亚铁硫杆菌氧化活性及浸出黄铜矿的影响[J]. 东北大学学报:自然科学版, 2016, 37(6): 861-865.
[3]	刘金亮，姜彬慧，赵鑫，胡筱敏. 一株高效絮凝剂产生菌的分离和鉴定[J]. 东北大学学报:自然科学版, 2016, 37(5): 706-710.
[4]	王有昭，朱彤，谢元华，李现瑾. 基于高通量测序技术的阳极生物膜群落结构[J]. 东北大学学报:自然科学版, 2016, 37(1): 105-109.
[5]	刘伟，杨洪英，佟琳琳，金哲男. 表面活性剂对钴矿石生物浸出的影响[J]. 东北大学学报:自然科学版, 2015, 36(6): 814-818.
[6]	李海军，杨洪英，陈国宝，罗文杰. 低品位硫铜钴矿生物浸出液中铜的分离[J]. 东北大学学报:自然科学版, 2014, 35(3): 391-395.
[7]	宋言，杨洪英，佟琳琳. 表面活性剂吐温80对钴精矿生物浸出的作用[J]. 东北大学学报:自然科学版, 2014, 35(12): 1750-1753.
[8]	刘伟，杨洪英，刘媛媛，罗文杰. 含钴矿石摇瓶生物浸出比较试验的研究[J]. 东北大学学报（自然科学版）, 2013, 34(11): 1606-1609.
[9]	李秀艳;周建民;魏德洲;郑龙熙. 金属硫化物矿物生物浸出过程中Fe~(3+)的作用[J]. 东北大学学报(自然科学版), 2001, 22(3): 291-294.