东北大学学报（自然科学版）

东北大学学报:自然科学版 ›› 2020, Vol. 41 ›› Issue (3): 452-456.DOI: 10.12068/j.issn.1005-3026.2020.03.026

• 生物工程 • 上一篇    

T形微流控芯片液滴成形与细胞封装的理论

胡晟， 廖子薇， 蔡露， 姜潇潇   

  1. (东北大学秦皇岛分校 控制工程学院， 河北 秦皇岛066004)
  • 收稿日期:2019-03-31 修回日期:2019-03-31 出版日期:2020-03-15 发布日期:2020-04-10
  • 通讯作者: 胡晟
  • 作者简介:胡晟(1984-)，男，云南景洪人，东北大学秦皇岛分校讲师，博士.
  • 基金资助:
    河北省自然科学基金资助项目(F2017501059，F2018501063)； 辽宁省博士启动基金资助项目(20170520325)； 中央高校基本科研业务费专项资金资助项目(N172304033).

Theory of Droplet Formation and Cell Encapsulation for T-Shaped Microfluidics Chip

HU Sheng， LIAO Zi-wei， CAI Lu， JIANG Xiao-xiao   

  1. School of Control Engineering， Northeastern University at Qinhuangdao， Qinhuangdao 066004， China.
  • Received:2019-03-31 Revised:2019-03-31 Online:2020-03-15 Published:2020-04-10
  • Contact: HU Sheng
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摘要： 连续相与离散相的速度取值是影响T形微流控芯片产生液滴的关键因素.细胞在T形微流通道的液滴包裹和细胞封装成为基因测序的研究热点，因此本文结合水平集与流体动力学方法，分析了液滴尺寸与红细胞封装的动态输运问题.在离散相和连续相通道宽度分别为50μm和80μm的T形微流控芯片内部，以水和正十二烷油为目标溶液进行计算仿真.计算结果表明水溶液初始速度0.012m/s，表面张力5mN/m，接触角165°时，毛细数Ca从0.008增大至0.1，会使液滴的尺寸降低，液滴成形频率加快.同时，黏滞力大于两相流表面张力会导致直径5μm红细胞的封装效率变低，使其紧贴通道墙壁作直线运动.

关键词: 微流控, 液滴, 细胞封装, 水平集, 流体动力学

Abstract: The velocity values of the continuous phase and the discrete phase are the key factors affecting the droplets generated by the T-shaped microfluidic chip. The droplet encapsulation and encapsulation of cells in T-shaped microfluidic channels become hot topics for gene sequencing. Therefore， the dynamic transport relative to both droplet size and blood cell encapsulation is studied by combining level set method with hydrodynamics. The water and n-dodecane oil as objective is simulated in the T-shaped microfluidics， in which the width of discrete and continuous channel is 50μm and 80μm， respectively. The results indicate that the capillary number (Ca) from 0.008 to 0.1 can result in decreasing droplet size and increasing generation frequency when there are the initial velocity of aqueous solution 0.012m/s， the interfacial tension 5mN/m and contact angle 165°. Meanwhile， the encapsulated efficiency can be decreased， so that the blood cell with diameter of 5μm moves straightway nearby the wall due to viscosity force greater than surface tension.

Key words: microfluidics, droplet, cell encapsulation, level set, hydrodynamics

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