大麦虫取食塑料影响因素及肠道功能菌群探究

doi:10.12068/j.issn.1005-3026.2021.07.016

东北大学学报（自然科学版） ›› 2021, Vol. 42 ›› Issue (7): 1019-1024.DOI: 10.12068/j.issn.1005-3026.2021.07.016

大麦虫取食塑料影响因素及肠道功能菌群探究

赵鑫^1,2，罗丽萍¹，王宇萌¹，郭鸿钦¹

(1.东北大学资源与土木工程学院，辽宁沈阳110819； 2.东北大学智慧水利与资源环境科技创新中心，辽宁沈阳110819)

修回日期:2020-10-11 接受日期:2020-10-11 发布日期:2021-07-16
通讯作者: 赵鑫
作者简介:赵鑫(1982-)，男，辽宁沈阳人，东北大学副教授，博士生导师.
基金资助:
“十三五”国家重点研发计划项目(2019YFC1907204)；辽宁省科技重大专项(2020020307-JH1/103-04)；中央高校基本科研业务费专项资金资助项目(N182508026).

Research on Influencing Factors of Polystyrene Consumption by Zophobas atratus Larvae， and Gut Core Functional Microbiome

ZHAO Xin^1,2， LUO Li-ping¹， WANG Yu-meng¹， GUO Hong-qin¹

1.School of Resources & Civil Engineering， Northeastern University， Shenyang 110819， China; 2.Science and Technology Innovation Center of Smart Water and Resource Environment， Northeastern University， Shenyang 110819， China.

Revised:2020-10-11 Accepted:2020-10-11 Published:2021-07-16
Contact: ZHAO Xin
About author:-
Supported by:
-

摘要/Abstract

摘要： 大麦虫取食降解泡沫塑料易受环境因子的影响而导致存活率降低.为了减少环境因子对塑料降解研究的干扰，通过条件实验探究了环境因素对大麦虫幼虫生长和取食泡沫塑料的影响，借助MiSeq测序解析了其消化道内潜在的塑料降解核心功能菌群.实验结果表明，光照、塑料密度与接触面积均对大麦虫生长和取食聚苯乙烯(Polystyrene，PS) 泡沫塑料有明显的影响.黑暗条件有利于大麦虫的生长和取食塑料；密度低且接触面积小的PS泡沫塑料更容易被大麦虫取食，但是破碎程度高的泡沫塑料不利于大麦虫的取食，而仅以PS泡沫塑料作为大麦虫的唯一食物无法满足其幼虫正常生长的需求.取食PS泡沫塑料对其消化道的菌群结构影响显著，unclassified Enterobacteriaceae，Dysgonomonas，Sphingobacterium和Enterococcus 4个菌属与PS泡沫塑料降解密切相关.

关键词: 塑料生物降解；大麦虫；光照条件；塑料密度；接触面积；功能菌群

Abstract: The survival rate of Zophobas atratus in the styrofoam biodegradation investigation is affected by environmental factors. In order to reduce the interference on the experiments of plastic biodegradation， effects of environmental factors on the growth of Zophobas atratus and consumption of the styrofoam were investigated， and the potential core functional bacteria was speculated by using the MiSeq sequencing technology. The experimental results indicated that light condition， plastic density and contact area have significant impacts on the growth of Zophobas atratus and the consumption of polystyrene(PS) styrofoam. Dark condition is conducive to the growth and plastic consumption by Zophobas atratus. Styrofoam with low density and low contact area is favored by Zophobas atratus. However， plastic with high breakage degree is not conducive to Zophobas atratus， and taking styrofoam as sole diet can not meet the growth requirements of larvae. Significant changes in gut microbiome of Zophobas atratus was observed with PS styrofoam diet， and genera of unclassified Enterobacteriaceae， Dysgonomonas， Sphingobacterium and Enterococcus are closely related to the degradation of PS styrofoam.

Key words: plastic biodegradation; Zophobas atratus; light condition; plastic density; contact area; functional microbiome

中图分类号:

X506

赵鑫，罗丽萍，王宇萌，郭鸿钦. 大麦虫取食塑料影响因素及肠道功能菌群探究[J]. 东北大学学报（自然科学版）, 2021, 42(7): 1019-1024.

ZHAO Xin， LUO Li-ping， WANG Yu-meng， GUO Hong-qin. Research on Influencing Factors of Polystyrene Consumption by Zophobas atratus Larvae， and Gut Core Functional Microbiome[J]. Journal of Northeastern University(Natural Science), 2021, 42(7): 1019-1024.

参考文献

[1]Blettler M C M，Nicolas G，Lea G，et al.Massive plastic pollution in a mega-river of a developing country:sediment deposition and ingestion by fish(Prochilodus lineatus)［J］.Environmental Pollution，2019，255:113348.
[2]Roy P K，Hakkarainen M，Varma I K，et al.Degradable polyethylene:fantasy or reality［J］.Environmental Science & Technology，2011，45:4217-4227.
[3]Law K L，Moret-ferguson S，Maximenko N A，et al.Plastic accumulation in the North Atlantic subtropical gyre［J］.Science，2010，329:1185-1188.
[4]Gwyer R，Jambeck J R，Law K L.Production，use，and fate of all plastics ever made［J］.Science Advances，2017，3:700782.
[5]Albertsson A C，Erlandsson B，Hakkarainen M，et al.Molecular weight changes and polymeric matrix changes correlated with the formation of degradation products in biodegraded polyethylene［J］.Journal of Polymers and the Environment，1998，6:187-195
[6]Horton A A，Walton A，Spurgeon D J，et al.Microplastics in freshwater and terrestrial environments:evaluating the current understanding to identify the knowledge gaps and future research priorities［J］.Science of the Total Environment，2017，586:127-141.
[7]郭鸿钦，罗丽萍，杨宇航，等.利用昆虫取食降解塑料研究进展［J］.应用与环境生物学报，2020，26:1546-1553.(Guo Hong-qin，Luo Li-ping，Yang Yu-hang，et al.Research progress on plastic degradation by worms［J］.Chinese Journal of Applied and Environmental Biology，2020，26:1546-1553.)
[8]Yang S S，Brandon A M，Flanagan J C A，et al.Biodegradation of polystyrene wastes in yellow mealworms(larvae of Tenebrio molitor Linnaeus):factors affecting biodegradation rates and the ability of polystyrene-fed larvae to complete their life cycle［J］.Chemosphere，2018，191:979-989.
[9]沈叶红.黄粉虫肠道菌的分离和取食塑料现象的研究［D］.上海:华东师范大学，2011.(Shen Ye-hong.Isolation of intestinal bacteria from Tenebrio molitor and study on the phenomenon of plastic degradation［D］.Shanghai:East China Normal University，2011.)
[10]Yang Y，Yang J，Wu W M，et al.Biodegradation and mineralization of polystyrene by plastic eating mealworms.Part l:chemical and physical characterization and isotopic tests［J］.Environmental Science & Technology，2015，49:12080-12086.
[11]陈冠舟，张白鹭，纪梦梦，等.高通量测序探究啮食聚苯乙烯泡沫塑料黄粉虫的肠道菌群结构［J］.微生物学通报，2017，44:2011-2018.(Chen Guan-zhou，Zhang Bai-lu，Ji Meng-meng，et al.Gut microbiota of polystyrene-eating mealworms analyzed by high-throughput sequencing［J］.Microbiology China，2017，44:2011-2018.)
[12]Brandon A M，Gao S H，Yang S S，et al.Biodegradation of polyethylene and plastic mixtures in mealworms(larvae of Tenebrio molitor) and effects on the gut microbiome［J］.Environmental Science & Technology，2018，52:6526-6533.
[13]Tschinkel W R.Zophobas atratus(Fab.) and Z.rugipes Kirsch(Coleoptera:Tenebrionidae) are the same species［J］.The Coleopterists Bulletin，1984，38:325-333.
[14]殷涛，周祥，王艳斌，等.泡沫塑料的取食对黄粉虫和大麦虫生长的影响［J］.甘肃农业大学学报，2018，53:74-79.(Yin Tao，Zhou Xiang，Wang Yan-bin，et al.Effects of plastic foam feeding on the growth of Tenebrio molitor L. and Zophobas morio Fabricius［J］.Journal of Gansu Agricultural University，2018，53:74-79.)
[15]苗少娟.大麦虫Zophobas morio的生物学特性及其对塑料降解作用的研究［D］.西安:西北农林科技大学，2010.(Miao Shao-juan.Study on biological characteristics of Zophobas morio and its function on plastic degradation［D］.Xi’an:Northwest Agriculture & Forestry University，2010.)
[16]杨莉，刘颖，高婕，等.大麦虫幼虫肠道菌群对聚苯乙烯泡沫塑料降解［J］.环境科学.2020，41:5609-5616.(Yang Li，Liu Ying，Gao Jie，et al.Biodegradation of expanded polystyrene foams in Zophobas morio:effects of gut microbiota［J］.Environmental Sciences，2020，41:5609-5616.)
[17]Yang J，Yang Y，Wu W M，et al.Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms［J］.Environmental Science & Technology，2014，48:13776-13784.
[18]周文宗，张硌，高红莉，等.温度和光线对黄粉虫蛹发育的影响［J］.现代农业科技，2006，10:124-125.(Zhou Wen-zong，Zhang Luo，Gao Hong-li，et al.Effects of temperature and light on pupa development of yellow mealworm［J］.Modern Agricultural Sciences and Technology，2006，10:124-125.)
[19]Peng B Y，Benbow M E，Criddle C S，et al.Biodegradation of polystyrene by dark(Tenebrio obscurus) and yellow(Tenebrio molitor) mealworms(Coleoptera:Tenebrionidae) ［J］.Environmental Science & Technology，2019，53:5256-5265.

大麦虫取食塑料影响因素及肠道功能菌群探究

Research on Influencing Factors of Polystyrene Consumption by Zophobas atratus Larvae， and Gut Core Functional Microbiome

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