一株高效絮凝剂产生菌的分离和鉴定

doi:10.12068/j.issn.1005-3026.2016.05.021

东北大学学报:自然科学版 ›› 2016, Vol. 37 ›› Issue (5): 706-710.DOI: 10.12068/j.issn.1005-3026.2016.05.021

一株高效絮凝剂产生菌的分离和鉴定

刘金亮，姜彬慧，赵鑫，胡筱敏

(东北大学资源与土木工程学院，辽宁沈阳110819)

收稿日期:2015-03-26 修回日期:2015-03-26 出版日期:2016-05-15 发布日期:2016-05-13
通讯作者: 刘金亮
作者简介:刘金亮(1984-)，男，山东枣庄人，东北大学博士研究生; 胡筱敏(1958-)，男，江西婺源人，东北大学教授，博士生导师.
基金资助:
国家自然科学基金资助项目 (51278090).

Isolation and Identification of an Efficient Bioflocculant-Producing Strain

LIU Jin-liang， JIANG Bin-hui， ZHAO Xin， HU Xiao-min

School of Resources & Civil Engineering， Northeastern University， Shenyang 110819， China.

Received:2015-03-26 Revised:2015-03-26 Online:2016-05-15 Published:2016-05-13
Contact: HU Xiao-min
About author:-
Supported by:
-

摘要/Abstract

摘要： 应用多相分类手段对絮凝菌株XHUA9进行了分类研究，并对分离提纯的发酵产物进行紫外和红外分析.基于16S rRNA基因序列的系统发育分析表明，该菌和模式菌株Paenibacillus hunanensis的序列同源性为96.7%，基因组DNA-DNA同源性杂交值为51.6%.菌株的主要极性脂肪酸为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇.菌株优势醌为MK-7，主要优势脂肪酸为anteiso-C_15:0和 C_16:0.菌株(G+C)摩尔分数为51.9%.根据上述结果，菌株XHUA9鉴定为类芽孢杆菌属内的新种，命名为Paenibacillus shenyangensis sp. nov..紫外扫描和红外检测等结果表明，菌株XHUA9所产絮凝剂的主要成分为多糖类物质.

关键词: 絮凝剂, 类芽孢杆菌, 鉴定, 16S rRNA, 多相分类

Abstract: In order to ascertain taxonomic position of strain XHUA9， the polyphasic taxonomical identification was performed. The ferment products were separated and purified and then were analyzed by ultraviolet scanning spectrum (UV scanning spectrum) and infrared spectrum (IR spectrum). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Paenibacillus， sharing a most closely sequence similarity of 96.7% and DNA-DNA hybridization value of 51.6% with the type strain Paenibacillus hunanensis. The major polar lipids were diphosphatidylglycerol， phosphatidylglycerol， phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinone is MK-7 and the major cellular fatty acids were anteiso-C_15:0 and C_16:0. The genomic DNA G+C content was 51.9 mol%. Based on the results of the present study， it is concluded that strain XHUA9 represents a novel species of the genus Paenibacillus， for which the name Paenibacillus shenyangensis sp. nov. is proposed. The UV scanning and IR spectra data showed that bioflocculant produced by strain XHUA9 was mainly composed of polysaccharides.

Key words: flocculant, Paenibacillus, identification, 16S rRNA, polyphasic taxonomical

中图分类号:

X172

刘金亮，姜彬慧，赵鑫，胡筱敏. 一株高效絮凝剂产生菌的分离和鉴定[J]. 东北大学学报:自然科学版, 2016, 37(5): 706-710.

LIU Jin-liang， JIANG Bin-hui， ZHAO Xin， HU Xiao-min. Isolation and Identification of an Efficient Bioflocculant-Producing Strain[J]. Journal of Northeastern University Natural Science, 2016, 37(5): 706-710.

参考文献

[1]马放，段姝悦，孔祥震，等.微生物絮凝剂的研究现状及其发展趋势［J］.中国给水排水，2012，28(2):14-17.(Ma Fang，Duan Shu-yue，Kong Xiang-zhen，et al.Present status and development trend of studies on microbial flocculants［J］.China Water & Wastewater，2012，28(2):14-17.)
[2]Sun J，Zhang X H，Miao X J，et al.Preparation and characteristics of bioflocculants from excess biological sludge［J］.Bioresource Technology，2012，126:362-366.
[3]Elkady M F，Farag S，Zaki S，et al.Bacillus mojavensis strain 32A，a bioflocculant-producing bacterium isolated from an Egyptian salt production pond［J］.Bioresource Technology，2011，102(17):8143-8151.
[4]Gong W X，Wang S G，Sun X F，et al.Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment［J］.Bioresource Technology，2008，99(11):4668-4674.
[5]Yang G，Wang N，Zhang C.Flocculating properties of the novel bioflocculant poly(γ-glutamic acid) and its use in jean dyeing wastewater treatment［J］.Advanced Materials Research，2011，183:125-129.
[6]Zhuang X L，Wang Y P，Li Q B，et al.The production of bioflocculants by Bacillus licheniformis using molasses and its application in the sugarcane industry［J］.Biotechnology and Bioprocess Engineering，2012，17(5):1041-1047.
[7]Ash C，Priest F G，Collins M D.Paenibacillus gen.nov.in validation of the publication of new names and new combinations previously effectively published outside the IJSB，list no.51［J］.International Journal of Systematic Bacteriology，1994，44:852-853.
[8]Berge O，Guinebretière M H，Achouak W，et al.Paenibacillus graminis sp.nov.and Paenibacillus odorifer sp.nov.，isolated from plant roots，soil and food［J］.International Journal of Systematic and Evolutionary Microbiology，2002，52(2):607-616.
[9]Jin H J，Lyu J，Chen S F.Paenibacillus sophorae sp.nov.，a nitrogen-fixing species isolated from the rhizosphere of Sophora japonica［J］.International Journal of Systematic and Evolutionary Microbiology，2011，61(4):767-771.
[10]Khianngam S，Tanasupawat S，Akaracharanya A，et al.Paenibacillus xylanisolvens sp.nov.，a xylan-degrading bacterium from soil［J］.International Journal of Systematic and Evolutionary Microbiology，2011，61(1):160-164.
[11]Kim D S，Bae C Y，Jeon J J，et al.Paenibacillus elgii sp.nov.，with broad antimicrobial activity［J］.International Journal of Systematic and Evolutionary Microbiology，2004，54(6):2031-2035.
[12]Collins M D.Isoprenoid quinone analysis in classification and identification［M］.London:Academic Press，1985:267-287.
[13]Zhao X，Xing D，Zhang L，et al.Characterization and over expression of a ［FeFe］-hydrogenase gene of a novel hydrogen-producing bacterium Ethanoligenens harbinense［J］.International Journal of Hydrogen Energy，2010，35(18):9598-9602.
[14]王秋红，蓝江林，朱育菁，等.脂肪酸甲酯谱图分析方法及其在微生物学领域的应用［J］.福建农业学报，2007，22(2):213- 218.(Wang Qiu-hong，Lan Jiang-lin，Zhu Yu-jing，et al.Fingerprint analysis on methyl fatty acid and its applications in microbial study［J］.Fujian Journal of Agricultural Sciences，2007，22(2):213- 218.)

[1]	姜彬慧，李凤达，刘倩，胡筱敏. 微生物絮凝剂MBFA9去除水中Pb²⁺的机理[J]. 东北大学学报:自然科学版, 2016, 37(11): 1588-1592.
[2]	付丽丽，姜彬慧，胡筱敏. Paenibacillus Shenyangensis的DNA序列拼接与组装[J]. 东北大学学报:自然科学版, 2016, 37(10): 1465-1468.
[3]	卢涛，魏德洲，张瑞洋，卓建英. 一株嗜酸氧化亚铁硫杆菌的鉴定及浸矿性能[J]. 东北大学学报:自然科学版, 2016, 37(1): 89-93.
[4]	王有昭，朱彤，谢元华，李现瑾. 基于高通量测序技术的阳极生物膜群落结构[J]. 东北大学学报:自然科学版, 2016, 37(1): 105-109.
[5]	鲁桂林;于海燕;迟松江;毕诗文;. 赤泥制备的聚合氯化铝铁处理高岭土废水[J]. 东北大学学报(自然科学版), 2010, 31(12): 1749-1752.
[6]	徐新阳;李立君;李茹;. 稠油炼化污水处理用聚合硅磷硫酸铁的制备[J]. 东北大学学报(自然科学版), 2009, 30(1): 137-140.
[7]	于长永;王国仁;毛克明;翟文丹;. 一种基于信息论的蛋白质数据库搜索鉴定算法[J]. 东北大学学报(自然科学版), 2009, 30(1): 50-53.
[8]	王一雍;张廷安;陈霞;鲍丽;. 后加矿增溶溶出赤泥矿浆沉降性能[J]. 东北大学学报(自然科学版), 2007, 28(10): 1430-1433.
[9]	吕子剑;毕诗文;谢雁丽;. 对苯二甲酸钠对赤泥沉降过程的影响[J]. 东北大学学报(自然科学版), 2007, 28(1): 83-86.
[10]	张廷安;豆志河;. 用壳聚糖脱除废水中的铜离子[J]. 东北大学学报(自然科学版), 2006, 27(2): 203-205.
[11]	张廷安;豆志河;. 壳聚糖絮凝剂处理废水中的Ag~+[J]. 东北大学学报(自然科学版), 2006, 27(1): 53-56.
[12]	胡筱敏;董怡华;李亮. 微生物絮凝剂BS-5的筛选及其特性[J]. 东北大学学报(自然科学版), 2005, 26(7): 691-694.
[13]	刘梅英;胡筱敏;张秀娟;苏永渤. 新型混凝剂聚合氯化铝钙的合成及其初步应用[J]. 东北大学学报(自然科学版), 2005, 26(5): 500-503.
[14]	张廷安;杨欢;赵乃仁;张国悦. 用壳聚糖絮凝剂处理含镉(Ⅱ)废水[J]. 东北大学学报(自然科学版), 2001, 22(5): 547-549.
[15]	邓述波;胡筱敏;罗茜. 高效生物絮凝剂的培养条件及特性[J]. 东北大学学报(自然科学版), 1999, 20(5): 525-528.

一株高效絮凝剂产生菌的分离和鉴定

Isolation and Identification of an Efficient Bioflocculant-Producing Strain

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价