Abstract: With a production line of large-scale series production taken as industrial background, the intelligent manufacturing technology of multi-agent and holonic manufacturing methodologies is applied to the construction of reconfigurable production line to improve the flexibility and reconfigurability of manufacturing system. Focusing on self-decision, the cooperation/coordination mechanism of intelligent entities in multi-agent and holonic manufacturing methodologies and the implementation/operation mechanism of manufacturing system are studied, as well as the application techniques in actual industrial background, e.g., the machining/assembly line which has been investigated by other researchers aiming at improving flexibility and reconfigurability. An assembly line of automotive transmission is taken as a case study from the viewpoint of planning and operating a production line, then the infrastructure construction by intelligent manufacturing cell and implementation methods in control, reconfigurable algorithms, product description and migration of system configuration are discussed.