Abstract: The preprocessing technique for a refractory gold concentrate in Hunan Province PRC was investigated experimentally, which contains 11.28% arsenic and its direct cyaniding leaching rate of gold is 21.91%. The occurrence form of arsenic in concentrate is mainly the arsenopyrite, and the gold-bearing minerals are arsenopyrite and pyrite, where the minutely dipped gold grains are wrapped up by arsenopyrite and pyrite. After pressure preoxidation, an experiment was done to extract gold from refractory gold concentrate by cyaniding leaching. The optimal conditions for pressure preoxidation are: Initial acidity is 1 mol/L, content of sodium lingosulfonates 5 g/t, ratio of HNO3 to H2SO4 is 7:1, pressure 0.6 MPa, stirring speed 300 rpm, processing time 100 min at 100°C and sampling pulp density 20%. The results showed that after the pressure preoxidation, the cyaniding leaching rate of gold comes to 90.87%, i.e., 68.96% is increased in comparison with the process without pressure preoxidation.