Abstract: Owing to the low content of membrane protein in cell and its complicated extraction/purification process with high cost, the membrane-related study on screening the non-membrane proteins which have strong affinity for biomembrane is of significance. With the IAM taken as a biomembrane model, it was filled into a capillary as the stationary phase in micro-column liquid chromatography. Then, ten kinds of common proteins were selected for investigating the interactions between IAM and then under acidic, neutral and alkali conditions. The results showed that the neutral phosphate buffer solution provides the environment where the proteins will combine best with IAM, thus enabling five kinds of proteins to provide strong affinity for IAM.