Abstract: An emulsion liquid membrane (ELM) process of Span80-TBP-NH3·H2O was presented for extraction of Nickel(II) from acidic leaching solution of laterite ore. The influence of temperature on the transport of Ni(II) through emulsion liquid membrane was studied, which showed that the emulsion liquid membrane(ELM) process of Span80-TBP-NH3·H2O was a chemical reaction controlling procedure. Demulsification experiments were carried out with ultrasonic-heating combined demulsification and heating-centrifugation combined emulsion breaking. The emulsion breaking rate may reach 98%. The membrane reactions in emulsion liquid membrane were investigated by FT-IR spectra method. The IR spectra comparison between the emulsions before and after nickel extraction confirmed the reactions in the membrane. The IR spectra comparison between the original oil and the oil after demulsification shows that the oil after demulsification can be reused. With such demulsified oil reused, the extraction rate of Nickel(II) from laterite ore can still reach 80% in the present pressure acid leaching-emulsion liquid membrane process.