Effects of C/N on the Denitrification Efficiency of SBR Enhanced by Aerobic Denitrifying Bacteria

doi:10.12068/j.issn.1005-3026.2018.08.028

Abstract

Abstract: Two strains of Pseudomonas with efficient aerobic denitrification ability were used in the bio-augmentation process of sequencing batch reactor. In the 61 days’ operating process， the effects of C/N on the microbial community structures and denitrification efficiency were investigated. Although the denitrification efficiencies obviously decreased with the C/N decreasing， the performances of bio-augmentation groups were better than those of the negative control. Adding efficient aerobic denitrifiers into activated sludge enhanced the NO^-₃-N removal rate by about 14.1%， whereas the pure-cultured group was enhanced by 22.6%. Bio-augmentation with aerobic denitrifiers enhanced the proportion of denitrifiers， promoted them to be the dominant functional population， shortened the system’s start-up period， greatly enhanced the denitrification efficiency， and improved the stability of the microbial community structure and the system’s operation. This technique is more advantageous in the treatment of C/N>10∶1 wastewater containing nitrate.

Key words: bio-augmentation, aerobic denitrification, SBR, denitrification efficiency, microbial community structure analysis

CLC Number:

X522

ZHAO Xin， LIU Fang， ZHAO Yan， HU Xiao-min. Effects of C/N on the Denitrification Efficiency of SBR Enhanced by Aerobic Denitrifying Bacteria[J]. Journal of Northeastern University Natural Science, 2018, 39(8): 1205-1210.

References

[1]中华人民共和国环境保护部.2014年中国环境状况公报［R］.北京:中华人民共和国环境保护部，2015.(PRC Ministry of Environmental Protection.Bulletin of Chinese state of environment in 2014 ［R］.Beijing:PRC Ministry of Environmental Protection，2015.)
[2]杨航.省界断面水质变化趋势及环境影响因子研究［D］.吉林:吉林大学，2015.(Yang Hang.Study on water quality trend and environmental influence factors in provincial section area ［D］.Jilin:Jilin University，2015.)
[3]Hossini H，Rezaee A，Ayati B，et al.Simultaneous nitrification and denitrification using a polypyrrole/microbial cellulose electrode in a membraneless bio-electrochemical system ［J］.RSC Advances，2015，5:72699-72708.
[4]Insel G，Erol S，vez S.Effect of simultaneous nitrification and denitrification on nitrogen removal performance and filamentous microorganism diversity of a full-scale MBR plant ［J］.Bioprocess and Biosystems Engineering，2014，37(11):2163-2173.
[5]Liu Y，Shi H，Xia L，et al.Study of operational conditions of simultaneous nitrification and denitrification in a carrousel oxidation ditch for domestic wastewater treatment ［J］.Bioresource Technology，2010，101(3):901-906.
[6]Wang X，Wang S，Xue T，et al.Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage ［J］.Water Research，2015，77:191-200.
[7]Gogina E，Gulshin I.Simultaneous nitrification and denitrification with low dissolved oxygen level and C/N ratio ［J］.Procedia Engineering，2016，153:189-194.
[8]Takaya N，Catalan-Sakairi M A，Sakaguchi Y，et al.Aerobic denitrification bacteria that produce low levels of nitrous oxide ［J］.Applied and Environmental Microbiology，2003，69(6):3152-3157.
[9]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法［M］.4版.北京:中国环境科学出版社，2002.(Editorial Board of Determination Methods for Examination of Water and Wastewater in SEPA.Determination methods for examination of water and wastewater［M］.4th ed.Beijing:China Environmental Science Press，2002.)
[10]Abu G O，Dike P O.A study of natural attenuation processes involved in a microcosm model of a crude oil-impacted wetland sediment in the Niger Delta ［J］.Bioresource Technology，2008，99(11):4761-4767.
[11]Falvo A，Levantesi C，Rossetti S，et al.Synthesis of intracellular storage polymers by Amaricoccus kaplicensis，a tetrad forming bacterium present in activated sludge ［J］.Journal of Applied Microbiology，2001，91(2):299-305.
[12]黄廷林，何秀秀，张海涵，等.水库贫营养异养硝化-好氧反硝化菌Sxf14的脱氮特性［J］.应用与环境生物学报，2015，21(2):201-207.(Huang Ting-lin，He Xiu-xiu，Zhang Hai-han，et al.Nitrogen removal characteristics of the heterotrophic nitrification-aerobic denitrification bacterium Acinetobacter sp.Sxf14 ［J］.Chinese Journal of Applied and Environmental Biology，2015，21(2):201-207.)
[13]张延青.颗粒污泥反硝化除磷优势菌种的分离与鉴定［D］.南京:南京农业大学，2012.(Zhang Yan-qing.Dominant strain of denitrification and phosphorus removal bacteria in granular sludge ［D］.Nanjing:Nanjing Agricultural University，2012.)
[14]Liu B，Mao Y，Bergaust L，et al.Strains in the genus Thauera exhibit remarkably different denitrification regulatory phenotypes ［J］.Environmental Microbiology，2013，15(10):2816-2828.
[15]Mao Y，Xia Y，Zhang T.Characterization of Thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial communities by using high-throughput sequencing ［J］.Bioresource Technology，2013，128:703-710.(上接第1195页)
[2]Liu W G，Wei D Z，Wang B Y，et al.A new collector used for flotation of oxide minerals［J］.Transactions of Nonferrous Metals Society of China，2009，19(5):1326-1330.
[3]Weng X Q，Mei G J，Zhao T T，et al.Utilization of novel ester-containing quaternary ammonium surfactant as cationic collector for iron ore flotation［J］.Separation & Purification Technology，2013，103(2):187-194.
[4]Huang Z Q，Zhong H，Wang S，et al.Comparative studies on flotation of aluminosilicate minerals with Gemini cationic surfactants BDDA and EDDA［J］.Transactions of Nonferrous Metals Society of China，2013，23(10):3055-3062.
[5]Jiang H，Liu G R，Hu Y H，et al.Flotation and adsorption of quaternary ammonium salts collectors on kaolinite of different particle size［J］.International Journal of Mining Science and Technology，2013，23(2):249-253.
[6]Filippov L O，Filippova I V，Severov V V.The use of collectors mixture in the reverse cationic flotation of magnetite ore:the role of Fe-bearing silicates［J］.Minerals Engineering，2010，23(2):91-98.
[7]Yao J，Yin W Z，Gong E P.Depressing effect of fine hydrophilic particles on magnesite reverse flotation［J］.International Journal of Mineral Processing，2016，149(4):84-93.
[8]Liu W B，Liu W G，Wei D Z，et al.Synthesis of N，N-Bis(2-hydroxypropyl)laurylamine and its flotation on quartz［J］.Chemical Engineering Journal，2017，309:63-69.
[9]熊道陵，李金辉，钟洪辉.应用基团电负性理论分析丙三醇黄原酸钠抑制毒砂作用机理［J］.中南大学学报(自然科学版)，2015，41(6):2156-2161.(Xiong Dao-ling，Li Jin-hui，Zhong Hong-hui.Analysis of suppression mechanism of arsenopyrite by glycerol sodium xanthogenate using group electronegativity theory［J］.Journal of Central South University (Science and Technology)，2015，41(6):2156-2161.)
[10]李明阳，魏德洲，崔宝玉，等.2，3-二巯基丁二酸对黄铜矿和辉钼矿浮选行为的影响［J］.东北大学学报(自然科学版)，2015，36(7):1020-1023.(Li Ming-yang，Wei De-zhou，Cui Bao-yu，et al.Flotation behavior of chalcopyrite and molybdenite in the presence of 2，3-Disulfanylbutanedioic Acid［J］.Journal of Northeastern University(Natural Science)，2015，36(7):1020-1023.)