Abstract: Pressure oxidation process was used to treat domestic refractory gold concentrate. The effects of reaction temperature, concentrate particle, oxygen partial pressure, initial acid concentration and reaction time on desulfurization rate and leaching rate of gold were investigated. Raw ore and residue were characterized by XRD, XRF, SEM and EDX. The desulfurization rate is 93.85% when the temperature is 180°C, concentrate particle size is -0.075~+0.061 mm, oxygen partial pressure is 0.8 MPa, initial acid concentration is 60 g/L, liquid-solid ratio is 4:1, reaction time is 120 min, and stirring speed is 600 r/min. The oxidation residue was used to extract gold using chlorination leaching (NaCl of 50 g/L and NaClO of 8 g/L). The leaching rate of gold is above 94.54% when the temperature is 30°C, pH arrangement is 4.5~5.0, liquid-solid ratio is 3:1, stirring speed is 300 r/min and reaction time is 180 min. The leaching rate of gold is 42.56% when the gold concentrate is not pretreated. Pretreatment plays an important role in rising leaching rate of gold.