Cloning and Expression Analysis of SOC1 Homologue Gene in Argyranthemum Frutescens

doi:10.12068/j.issn.1005-3026.2021.04.021

Abstract

Abstract: SOC1 is one of crucial genes in integrating several pathway signals to initiate the transition of flowering. The SOC1 homologue gene AfSOC1 was identified and cloned from Argyranthemum frutescens using RT-PCR and its expression patterns were characterized using qRT-PCR. The results show that AfSOC1 gene consists of a 648-bp open reading frame and encodes a putative protein of 216 amino acids， which is 63.1% identical to SOC1 in Arabidopsis and 62.1% to DEFH68 in Antirrhinum. The putative protein sequences have a typical MADS-box， K-box domain and typical SOC1 motif DVETELFIGP. AfSOC1 was expressed at highly levels in leaves and flower bud. Its expression signals were also detected in shoot， stems， and flowers. Phylogenetic analysis showed that AfSOC1 was most closely related to dicotyledon SOC1-like than to monocotyledon. These results laid a foundation for us to understand the mechanism of AfSOC1 in regulation flowering.

Key words: Argyranthemum frutescens; SOC1 homologue gene; qRT-PCR; cloning， molecular phylogeny

CLC Number:

TP20

MA Yue-ping， HU Jing. Cloning and Expression Analysis of SOC1 Homologue Gene in Argyranthemum Frutescens[J]. Journal of Northeastern University(Natural Science), 2021, 42(4): 604-608.

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