Abstract: To understand in depth how low temperature affects the deformation behavior of the laminated seismic isolation devices made of rubber-steel plate, two kinds of those isolators were investigated experimentally in Shenyang where the weather is quite cold in winter for comparison. The test proved that low temperature affects prominently the vertical compressive stiffness, while its effect on horizontal stiffness varies with the quality and manufacturing process of the laminated rubber. Moreover, the horizontal stiffness rises with increasing compressive stress, with the horizontal stiffness variabilities of the two laminated rubber isolators under to-and-fro horizontal load revealed. An advice is thus given that the vertical compressive stiffness and horizontal stiffness should be taken into account when designing and using laminated rubber isolators.