Abstract: Several common associated minerals in refractory gold ore were tested to analyze their effects on cyanide leaching. The effects of Fe²⁺, Cu²⁺, As³⁺ on the consumption of cyanide were analysed by standard solution of chemical reagents. The results showed that pyrrhotite has the greatest effect on cyanide leaching in iron minerals, which decreases gold dissolution rate by 28.1% and increases cyanide consumption by 4 times. Pyrite and hematite have little effects. Chalcopyrite and chalcocite have great effects on cyanide leaching in copper minerals. Chalcocite decreases gold dissolution rate by 36.81% and increases cyanide consumption by 10 times. Realgar and orpiment are extremely harmful to cyanide leaching in arsenic minerals, which decreases gold dissolution rate by 41.95% and 49.90% respectively, and increases cyanide consumption by 13.8 times and 15.0 times. On the contrary, arsenopyrite is more stable in the system, and has little effect on cyanide leaching. In ion consuming cyanide test, Fe²⁺ and Cu²⁺ consume a lot of cyanide in the system, while As³⁺ isn't reactive with cyanide and cannot cause cyanide consumption. Thus the formation of thio-cyanate is the main reason of arsenic minerals consuming a lot of cyanide.