Abstract: The velocity values of the continuous phase and the discrete phase are the key factors affecting the droplets generated by the T-shaped microfluidic chip. The droplet encapsulation and encapsulation of cells in T-shaped microfluidic channels become hot topics for gene sequencing. Therefore， the dynamic transport relative to both droplet size and blood cell encapsulation is studied by combining level set method with hydrodynamics. The water and n-dodecane oil as objective is simulated in the T-shaped microfluidics， in which the width of discrete and continuous channel is 50μm and 80μm， respectively. The results indicate that the capillary number (C_a) from 0.008 to 0.1 can result in decreasing droplet size and increasing generation frequency when there are the initial velocity of aqueous solution 0.012m/s， the interfacial tension 5mN/m and contact angle 165°. Meanwhile， the encapsulated efficiency can be decreased， so that the blood cell with diameter of 5μm moves straightway nearby the wall due to viscosity force greater than surface tension.

Key words: microfluidics, droplet, cell encapsulation, level set, hydrodynamics