Abstract: TiO2 nano-film with homogeneous surface was prepared on the glass substrate by the sol-gel method. The film was characterized by SEM and XRD. The adsorption of trace Cu(II) was carried out taking TiO2 nano-film as an adsorbent. The effects of pH values, adsorption time, initial concentrations of Cu(II) and coexistent ions on the adsorption rates were studied systematically. The results showed that the film is composed of anatase TiO2 nanoparticles with the grain diameter about 50 nm. The adsorption isotherm of Cu(II) on TiO2 nano-film shows "L" type, which is explained by the monolayer adsorption. The Cu(II) adsorbed on TiO2 nano-film is eluted by 1.0 mol/L nitric acid efficiently. The optimum adsorption conditions are determined.