Abstract: S-wave velocity is significant to the determination of dynamic parameters and evaluation of rockmass stability. A new measurement method of S-wave velocity for small-size rock samples is put forward according to the propagating law of elastic wave. The S-wave velocity can be measured with the probes for P-wave by the V-shaped groove on the bottom of specimen to eliminate the interference from P-wave. The S-wave velocities of rock samples with layered structure are thus obtained with the anisotropic coefficient of S-wave velocity calculated. The results showed that the change of S-wave velocity in rock is similar to that of P-wave velocity, i.e., observing the law of elliptic equation. It was proved that the new measurement method is applicable via the analysis of the ratio of P-wave velocity to S-wave velocity.