Abstract: PHA and rhIL2 were adopted to stimulate human peripheral blood lymphocytes. The cell cycle and cell proliferation were analyzed based on the flowing cytometry (FCM). By using TRAP， the changes of telomerase activity in peripheral blood lymphocytes before and after activation were detected. By using Western blot， the expression of hTERT and related protein were detected. By using the fluorescent quantitative PCR， the mRNA levels of hTERT were analyzed. The results showed that after the peripheral blood lymphocytes were activated by PHA and rhIL2， telomerase activity was elevated， and elevated telomerase activity could be restrained by the JAK inhibitor. These results indicated that the increase of the telomerase activity depends on JAK signal pathway. Elevated telomerase activity is due to the increase of hTERT protein expression， while the hTERT is due to the increase of mRNA expression levels of hTERT. The results of the experiments showed that these activities also depends on the JAK signal pathway. Evidences about the telomerase activity and hTERT regulation mechanism are provided based on this study.

Key words: telomerase activity, lymphocyte, hTERT, mRNA, JAK signal pathway