Abstract: During the establishment of simulation platform， the electron microscope cartilage tomographic image stitching is one of the key technologies. The twodimensional electron microscope method was studied. Firstly， the SIFT algorithm was used to detect key points in the image， and the kdtree was used to create data index to match the key points fast， then the image shift could be determined. Finally， the seamless stitching of partial slicing image was realized and the problem of small angle for electron microscope image was solved effectively. C language was used in VS2010 environment for simulation. The experimental results showed that the method presented is robust and realtime. It was proved that this method could provide objective scientific support in the cultivation process of artificial cartilage tissue.

Key words: cartilage, electron microscope image, image stitching, SIFT, kdtree