Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan

doi:10.12068/j.issn.1005-3026.2016.01.019

Journal of Northeastern University Natural Science ›› 2016, Vol. 37 ›› Issue (1): 89-93.DOI: 10.12068/j.issn.1005-3026.2016.01.019

• Resources & Civil Engineering • Previous Articles Next Articles

Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan

LU Tao¹， WEI De-zhou¹， ZHANG Rui-yang¹， ZHUO Jian-ying²

1.School of Resources & Civil Engineering， Northeastern University， Shenyang 110819，China; 2. Team 403 in Liaoning Provincial Bureau of Metallurgical & Geological Prospecting， Anshan 114018， China.

Received:2014-11-10 Revised:2014-11-10 Online:2016-01-15 Published:2016-01-08
Contact: LU Tao
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Abstract

Abstract: A bioleaching bacterium was isolated from an acidic mine drainage of Jiama polymetallic mine in Tibet and was named as XZ. The strain XZ， with round ends， is gram-negative and short-rod shaped in 1～1.5μm×0.5μm. Ferrous iron and elemental sulfur could be oxidized by strain XZ. The optimum growth pH is 2.0 and the optimum growth temperature is 30℃. It was identified as Acidithiobacillus ferrooxidans by means of growth characteristic research and 16S rRNA sequencing. The strain XZ was used to leach a poor copper hornstone ore took from Tibet.Shaking flask leaching tests were carried out with inoculation size of 10%(volume fraction)， pulp mass fraction of 10% ，ore particle size of -45μm accounting for 100%， leaching temperature of 30℃ and shaking intensity of 160r/min. The results show that leaching rate of copper can reach 72.15% after 15 days.

Key words: Acidithiobacillus ferrooxidans, strain identification, 16S rRNA, bioleaching, poor copper ore

CLC Number:

TD925.5
Q939

LU Tao， WEI De-zhou， ZHANG Rui-yang， ZHUO Jian-ying. Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan[J]. Journal of Northeastern University Natural Science, 2016, 37(1): 89-93.

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Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan

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