Isolation and Identification of an Efficient Bioflocculant-Producing Strain

doi:10.12068/j.issn.1005-3026.2016.05.021

Abstract

Abstract: In order to ascertain taxonomic position of strain XHUA9， the polyphasic taxonomical identification was performed. The ferment products were separated and purified and then were analyzed by ultraviolet scanning spectrum (UV scanning spectrum) and infrared spectrum (IR spectrum). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Paenibacillus， sharing a most closely sequence similarity of 96.7% and DNA-DNA hybridization value of 51.6% with the type strain Paenibacillus hunanensis. The major polar lipids were diphosphatidylglycerol， phosphatidylglycerol， phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinone is MK-7 and the major cellular fatty acids were anteiso-C_15:0 and C_16:0. The genomic DNA G+C content was 51.9 mol%. Based on the results of the present study， it is concluded that strain XHUA9 represents a novel species of the genus Paenibacillus， for which the name Paenibacillus shenyangensis sp. nov. is proposed. The UV scanning and IR spectra data showed that bioflocculant produced by strain XHUA9 was mainly composed of polysaccharides.

Key words: flocculant, Paenibacillus, identification, 16S rRNA, polyphasic taxonomical

CLC Number:

X172

LIU Jin-liang， JIANG Bin-hui， ZHAO Xin， HU Xiao-min. Isolation and Identification of an Efficient Bioflocculant-Producing Strain[J]. Journal of Northeastern University Natural Science, 2016, 37(5): 706-710.

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