Journal of Northeastern University Natural Science ›› 2016, Vol. 37 ›› Issue (1): 105-109.DOI: 10.12068/j.issn.1005-3026.2016.01.022

• Resources & Civil Engineering • Previous Articles     Next Articles

Community Structure of Anode Biofilm by High-Throughput Sequencing Technology

WANG You-zhao,ZHU Tong,XIE Yuan-hua,LI Xian-jin   

  1. School of Mechanical Engineering & Automation,Northeastern University,Shenyang 110819,China.
  • Received:2014-10-27 Revised:2014-10-27 Online:2016-01-15 Published:2016-01-08
  • Contact: ZHU Tong
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Abstract: Microbial community structure of anode biofilm was analyzed using high-throughput sequencing technology. The results show that the technology can obtain community structure information from electrochemistry biofilm efficiently, and the exoelectrogentic modal bacteria Geobacter category is predominant in biological membrane, its quantity ratio reaches 30%;Thermovirga,Thauera and Syntrophorhabdus are also found in anode biofilm. However, through comparison of biofilm before and after electrochemical activity was lost, Geobacter still dominated in biofilm after the biofilm lost electrochemical activity,while Actinomycetes’ proportion increased significantly, its quantity was much higher than that of the normal 0.5%. So Actinomycetes may be one of the factors which inhibit the electrochemical activity of the biofilm.

Key words: anode biofilm, bioelectrochemical system (BES);high-throughput sequencing, microbial diversity, 16S rRNA

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