Journal of Northeastern University Natural Science ›› 2016, Vol. 37 ›› Issue (10): 1465-1468.DOI: 10.12068/j.issn.1005-3026.2016.10.021

• Resources & Civil Engineering • Previous Articles     Next Articles

DNA Sequence Assembly of Paenibacillus Shenyangensis

FU Li-li1,2, JIANG Bin-hui1, HU Xiao-min1   

  1. 1. School of Resources & Civil Engineering, Northeastern University, Shenyang 110819, China; 2. College of Petroleum Engineering, Liaoning Shihua University, Fushun 113001, China.
  • Received:2015-10-27 Revised:2015-10-27 Online:2016-10-15 Published:2016-10-14
  • Contact: HU Xiao-min
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Abstract: On the basis of existing sequencing data, three sequence assembling programs were utilized to assemble the genome sequence of Paenibacillus Shenyangensis. The assembly data of DNA sequence were analyzed and compared with different software in their optimal parameters, and were compared with genes of the other species of the Paenibacillus in the NCBI database.The results showed that SOAPdenovo is the most appropriate assemble software. When k-mer is 23, the total genome length and N50 are 5501467 and 293864bp, respectively. 4393 of the total 4800 genes are successfully matched and annotated.

Key words: sequence assembly, genome, flocculants bacteria, high-throughput sequencing, Paenibacillus

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