Journal of Northeastern University Natural Science ›› 2018, Vol. 39 ›› Issue (5): 693-698.DOI: 10.12068/j.issn.1005-3026.2018.05.018

• Resources & Civil Engineering • Previous Articles     Next Articles

Evolution Analysis of Microbial Community During the Running Process in an ANAMMOX Reactor

ZHU Tong, JIA Ruo-tan, LIANG Qi-yu, XIE Yuan-hua   

  1. School of Mechanical Engineering & Automation, Northeastern University, Shenyang 110819, China.
  • Received:2016-12-20 Revised:2016-12-20 Online:2018-05-15 Published:2018-05-25
  • Contact: ZHU Tong
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Abstract: In order to provide theoretical support for the optimization of anaerobic ammonium oxidation (ANAMMOX)technology, high-throughput sequencing technology was used to study the microbial community structure and its evolution in an ANAMMOX reactor. The results showed that the reactor achieved nitrogen removal rate up to 1.04kg·N·m-3·d-1 after 61 days. The total nitrogen removal rate was above 75%.The microbial diversity in the reactor was very abundant,including Proteobacteria,Chloroflexi, Chlorobi,Planctomycetes, etc.The microbial community structure tended to be stable with the running process of the reactor.The proportion of Proteobacteria and Planctomycete, associated with denitrification, totally achieved over 40%. The proportion of ANAMMOX bacteria belonging to Planctomycete increased gradually with reactor operation, which was the main reason for denitrification efficiency improvement.The ANAMMOX bacteria Candidatus Kuenenia category dominated in reactor about 14%. A small number of Candidatus Brocadia genus also existed in the reactor.

Key words: ANAMMOX(anaerobic ammonium oxidation), high-throughput sequencing, community characteristics, denitrification, microbe

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