In order to extract pure cell populations from multiple cell populations or to extract the required components from complex samples, a novel regulation method of induced?charge electro?osmotic (ICEO) is proposed, based on the superposition effect of dual electric fields, to study the remodeling mechanism of the ICEO vortex and its particle control performance. Firstly, a multi?physical coupling simulation model is established and the asymmetric evolution mechanism is studied. Secondly, the particle control device is designed and processed, and the particle control experimental system is built. Then, the aggregation and longitudinal migration characteristics of single particle induced by asymmetrically ICEO vortices at different voltages are studied. Finally, aggregation and separation characteristics of various particles within the asymmetric ICEO vortices are explored. The results show that this method can achieve the aggregation, migration and separation of micro?scale particles in a simple control way, and it has great application potential in the field of environmental detection and disease diagnosis.